-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-Shared dataset for the publication: "The Effect of Acute Pharmacological Inhibition of Urokinase Plasminogen Activator and Neuropsin Extracellular Proteases on Neuronal Networks in vitro" Authored by Sebastiaan Van De Vijver, Stephan Missault, Jeroen Van Soom, Pieter Van Der Veken, Koen Augustyns, Jurgen Joossens, Stefanie Dedeurwaerdere, and Michele Giugliano -=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=- 1. Content of ProcessedData.zip ------------------------------- - All the data is organised in 2 main folders: Treated (i.e., treated with a uPA/KLK8 inhibitor in DMSO solution) and Control (i.e., treated with DMSO solution only). - Each of these two folders contains 3 subfolders: Baseline (i.e. recorded before administration of the solution), LowConcentration (i.e. recorded 10 minutes after adding a low dosage), HighConcentration (i.e. recorded 10 minutes after adding additional solution to reach a high final dosage). ProcessedData ├── Control │   ├── Baseline │   │   ├── 201508131112_I7880_div27_spikes.txt │   │   ├── 201508141318_I6985_div30_spikes.txt │   │   ├── 201508141318_I7303_div29_spikes.txt │   │   ├── 201605251042_I9888_div28_spikes.txt │   │   ├── 201605251052_I8422_div28_spikes.txt │   │   ├── 201605251506_I8023_div28_spikes.txt │   │   └── 201605251506_I8137_div28_spikes.txt │   ├── HighConcentration │   │   ├── 201508131337_I7880_div27_spikes.txt │   │   ├── 201508141538_I6985_div30_spikes.txt │   │   ├── 201508141538_I7303_div29_spikes.txt │   │   ├── 201605251319_I8422_div28_spikes.txt │   │   ├── 201605251319_I9888_div28_spikes.txt │   │   ├── 201605251745_I8137_div28_spikes.txt │   │   └── 201605251746_I8023_div28_spikes.txt │   └── LowConcentration │   ├── 201508131226_I7880_div27_spikes.txt │   ├── 201508141429_I6985_div30_spikes.txt │   ├── 201508141429_I7303_div29_spikes.txt │   ├── 201605251205_I8422_div28_spikes.txt │   ├── 201605251205_I9888_div28_spikes.txt │   ├── 201605251633_I8023_div28_spikes.txt │   └── 201605251633_I8137_div28_spikes.txt └── Treated ├── Baseline │   ├── 201508121023_I7490_div28_spikes.txt │   ├── 201508121023_I7496_div28_spikes.txt │   ├── 201508121410_I7493_div28_spikes.txt │   ├── 201508121410_I7512_div28_spikes.txt │   ├── 201508131003_I6992_div28_spikes.txt │   ├── 201508131537_I7608_div30_spikes.txt │   ├── 201508140949_I8023_div29_spikes.txt │   ├── 201508140950_I7519_div29_spikes.txt │   ├── 201605271058_I6607_div30_spikes.txt │   └── 201605271550_I6878_div30_spikes.txt ├── HighConcentration │   ├── 201508121250_I7490_div28_spikes.txt │   ├── 201508121250_I7496_div28_spikes.txt │   ├── 201508121655_I7493_div28_spikes.txt │   ├── 201508121655_I7512_div28_spikes.txt │   ├── 201508131226_I6992_div28_spikes.txt │   ├── 201508131553_I7608_div30_spikes.txt │   ├── 201508141212_I7519_div29_spikes.txt │   ├── 201508141212_I8023_div29_spikes.txt │   ├── 201605271326_I6607_div30_spikes.txt │   └── 201605271824_I6878_div30_spikes.txt └── LowConcentration ├── 201508121134_I7490_div28_spikes.txt ├── 201508121134_I7496_div28_spikes.txt ├── 201508121542_I7493_div28_spikes.txt ├── 201508121542_I7512_div28_spikes.txt ├── 201508131112_I6992_div28_spikes.txt ├── 201508131445_I7608_div30_spikes.txt ├── 201508141100_I7519_div29_spikes.txt ├── 201508141100_I8023_div29_spikes.txt ├── 201605271210_I6607_div30_spikes.txt └── 201605271700_I6878_div30_spikes.txt 8 directories, 51 files - Each subfolder contains several ASCII text (*.txt) files, named according to the following convention: RecordedDateTime_MEA-ID_CultureAgeInDaysInVitro_spikes.txt where the "RecordedDateTime" is e.g. 2015 08 14 1318, the "MEA-ID"entifier is e.g. I7303, the "CultureAgeInDaysInVitro" is e.g. div29, and contains the (spontaneous) spikes detected at each of the 60 microelectrode of that MEA, over 1 hour of continuous recordings. The content of each file is a space-separated values (%f %f)of the type: time_of_the_spike_detected(in_ms) unique_ID_of_the_microelectrode_which_detected_it A simple script in MATLAB can be used to open the file and plot it: - data = load('201605251052_I8422_div28_spikes.txt'); - time = data(:,1); - indx = data(:,2); - plot(time, indx, 'o'); - xlim([250000 260000]); 2. Content of RawData.zip ------------------------- Sample raw voltage recordings (*.mcd file format - MultiChannel Systems - see https://www.multichannelsystems.com/software/mc-datatool) - 258_c_I7490_div28_spont_201508121023.mcd Spontaneous "baseline" activity - 258_c_I7490_div28_upai10um_201508121251.mcd Spontaneous activity under 10microM concentration 3. Content of Code.zip ---------------------- MATLAB custom code scripts to perform post processing of the spike detected data: Main.m main_cc.m CC.m burst_alignment.m burst_detection.m find_burst_duration.m pairwise_cc.m populated_fig.m