FastQCFastQC Report
H 27 aug. 2018
Human_alphaherpesvirus_3_NC_001348_CAP.sam.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameHuman_alphaherpesvirus_3_NC_001348_CAP.sam.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences509531
Sequences flagged as poor quality0
Sequence length56-5793
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTTGTACTTCGTTCAGTTACGTATTGCTTCTCAGGCGTTTTTTTTTTT17300.33952791881161304No Hit
TTGTTGTACTTCGTTCAGTTACGTATTGCTTGGATTGATATGTAATACGA15120.2967434758630976No Hit
TTGTTATTACTTCGTTCAGTTACGTATTGCTTCTCAGGCGTTTTTTTTTT9230.18114697633706292No Hit
TTGGTATTACTTCGTTCAGTTACGTATTGCTTCTCAGGCGTTTTTTTTTT7870.15445576422239274No Hit
TTGGTATGCTTCGTTCAGTTACGTATTGCTTCTCAGGCGTTTTTTTTTTT7590.1489605146693724No Hit
TTGGTATTACTTCGTTCAGTTACGTATTGCTTGGATTGATATGTAATACG7490.14699792554329374No Hit
TTGGTAATACTTCGTTCAGTTACGTATTGCTTCTCAGGCGTTTTTTTTTT7410.14542785424243077No Hit
TTGGTATGCTTCGTTCAGTTACGTATTGCTTGGATTGATATGTAATACGA6430.12619448080685966No Hit
TTGTTATTACTTCGTTCAGTTACGTATTGCTTGGATTGATATGTAATACG6200.12168052581687866No Hit
TTGGTACTTCGTTCAGTTACGTATTGCTTGGATTGATATGTAATACGACT6020.11814786538993702No Hit
TTATTGTACTTCGTTCAGTTACGTATTGCTTCTCAGGCGTTTTTTTTTTT5660.11108254453605375No Hit
TTGGTAATACTTCGTTCAGTTACGTATTGCTTGGATTGATATGTAATACG5480.1075498841091121No Hit
TTGGTACTTCGTTCAGTTACGTATTGCTTCTCAGGCGTTTTTTTTTTTTT5400.10597981280824914No Hit
TTATTGTACTTCGTTCAGTTACGTATTGCTTGGATTGATATGTAATACGA5200.10205463455609178No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTTT49500.0326.76411
GTTGTAC21800.0304.55053
TGGTACT10500.0286.59712
TTGGTAT32250.0278.76571
TGCGGGG13300.0264.442381
TTGTACT35600.0264.156374
TTGGTAC12300.0259.947051
TTGGTAG7250.0256.82561
TGGTATG15150.0245.806662
TGCAAGT3900.0241.127371
GCGGTTA11200.0235.099172
TGTTATT17500.0234.29312
CGGGGTT13700.0229.264593
TGTACTT50800.0228.805185
CCCGCTA8100.0222.908841
CCCGTTA2150.0218.696911
CTTTTTA7500.0208.141142
TGCGTGT5700.0204.577511
TGTTGTA33300.0202.764222
GGTATGC22050.0202.15333
Produced by FastQC (version 0.11.5)