<i>De Novo</i> Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles

Published on 2018-03-13T13:36:04Z (GMT) by
Cell division is the most dynamic event in the cell cycle. Recently, efforts have been made to reconstruct it using the individual component proteins to obtain a better understanding of the process of self-reproduction of cells. However, such reconstruction studies are frequently hampered by difficulties in preparing membrane-associated proteins. Here we demonstrate a <i>de novo</i> synthesis approach based on a cell-free translation system. Genes for fundamental cell division proteins, FtsZ, FtsA, and ZipA, were expressed inside the lipid compartment of giant vesicles (GVs). The synthesized proteins showed polymerization, membrane localization, and eventually membrane deformation. Notably, we found that this morphological change of the vesicle is forced by only FtsZ and ZipA, which form clusters on the membrane at the vesicle interior. Our cell-free approach provides a platform for studying protein dynamics associated with lipid membrane and paves the way to create a synthetic cell that undergoes self-reproduction.

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Furusato, Takumi; Horie, Fumihiro; Matsubayashi, Hideaki T.; Amikura, Kazuaki; Kuruma, Yutetsu; Ueda, Takuya (2018): De Novo Synthesis of Basal Bacterial

Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles. ACS Publications. Collection.