Three-Dimensional
Localization of an Individual Fluorescent
Molecule with Angstrom Precision
Posted on 2017-06-22 - 21:04
Among
imaging techniques, fluorescence microscopy is a unique method
to noninvasively image individual molecules in whole cells. If the
three-dimensional spatial precision is improved to the angstrom level,
various molecular arrangements in the cell can be visualized on an
individual basis. We have developed a cryogenic reflecting microscope
with a numerical aperture of 0.99 and an imaging stability of 0.05
nm in standard deviation at a temperature of 1.8 K. The key optics
to realize the cryogenic performances is the reflecting objective
developed by our laboratory. With this cryogenic microscope, an individual
fluorescent molecule (ATTO647N) at 1.8 K was localized with standard
errors of 0.53 nm (x), 0.31 nm (y), and 0.90 nm (z) when 106 fluorescence
photons from the molecule were accumulated in 5 min.
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Furubayashi, Taku; Motohashi, Kazuya; Wakao, Keisuke; Matsuda, Tsuyoshi; Kii, Isao; Hosoya, Takamitsu; et al. (2017). Three-Dimensional
Localization of an Individual Fluorescent
Molecule with Angstrom Precision. ACS Publications. Collection. https://doi.org/10.1021/jacs.7b03899
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AUTHORS (11)
TF
Taku Furubayashi
KM
Kazuya Motohashi
KW
Keisuke Wakao
TM
Tsuyoshi Matsuda
IK
Isao Kii
TH
Takamitsu Hosoya
NH
Nobuhiro Hayashi
MS
Mahito Sadaie
FI
Fuyuki Ishikawa
MM
Michio Matsushita
SF
Satoru Fujiyoshi