Thermomechanical Response
of a Representative Porin
for Biomimetics
Version 2 2018-07-13, 17:25
Version 1 2018-07-13, 17:19
Posted on 2018-07-13 - 17:25
The
thermomechanical response of Omp2a, a representative porin
used for the fabrication of smart biomimetic nanomembranes, has been
characterized using microcantilever technology and compared with standard
proteins. For this purpose, thermally induced transitions involving
the conversion of stable trimers to bigger aggregates, local reorganizations
based on the strengthening or weakening of intermolecular interactions,
and protein denaturation have been detected by the microcantilever
resonance frequency and deflection as a function of the temperature.
Measurements have been carried out on arrays of 8-microcantilevers
functionalized with proteins (Omp2a, lysozyme and bovine serum albumin).
To interpret the measured nanofeatures, the response of proteins to
temperature has been also examined using other characterization techniques,
including real time wide angle X-ray diffraction. Results not only
demonstrate the complex behavior of porins, which exhibit multiple
local thermal transitions before undergoing denaturation at temperatures
higher than 105 °C, but also suggest a posttreatment to control
the orientation of immobilized Omp2a molecules in functionalized biomimetic
nanomembranes and, thus, increase their efficacy in ion transport.
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Lopes-Rodrigues, Maximilien; Puiggalí-Jou, Anna; Martí-Balleste, Didac; J. del Valle, Luis; Michaux, Catherine; Perpète, Eric A.; et al. (2018). Thermomechanical Response
of a Representative Porin
for Biomimetics. ACS Publications. Collection. https://doi.org/10.1021/acsomega.8b00463
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AUTHORS (7)
ML
Maximilien Lopes-Rodrigues
AP
Anna Puiggalí-Jou
DM
Didac Martí-Balleste
LJ
Luis J. del Valle
CM
Catherine Michaux
EP
Eric A. Perpète
CA
Carlos Alemán
KEYWORDS
angle X-ray diffractionOmp 2amicrocantilever technologybiomimetic nanomembranescharacterization techniquesion transportOmp 2a lysozymethermomechanical responseOmp 2a moleculesfunctionalized biomimetic nanomembranesmicrocantilever resonance frequencyserum albuminRepresentative Porinprotein denaturationtransitionrepresentative porinThermomechanical Response8- microcantilevers functionalized