Synthesis and Characterization of Fatty Acid Grafted
Chitosan Polymer and Their Nanomicelles for Nonviral Gene Delivery
Applications
Version 2 2017-10-23, 13:09
Version 1 2017-10-23, 13:05
Posted on 2017-10-23 - 13:09
The aim of this study
was to synthesize and characterize fatty
acid-grafted-chitosan (fatty acid-g-CS) polymer and
their nanomicelles for use as carriers for gene delivery. CS was hydrophobically
modified using saturated fatty acids of increasing fatty acyl chain
length. Carbodiimide along with N-hydroxysuccinimide
was used for coupling carboxyl group of fatty acids with amine groups
of CS. Proton nuclear magnetic resonance and Fourier transform infrared
spectroscopy were used to quantify fatty acyl substitution onto CS
backbone. The molecular weight distribution of the synthesized polymers
was determined using size exclusion high performance liquid chromatography
and was found to be in range of the parent CS polymer (∼50
kDa). The critical micelle concentration (cmc) of the polymers was
determined using pyrene as a fluorescent probe. The cmc was found
to decrease with an increase in fatty acyl chain length. The amphiphilic
fatty acid-g-CS polymers self-assembled in an aqueous
environment to form nanomicelles of ∼200 nm particle size and
slightly positive net charge due to the cationic nature of free primary
amino groups on CS molecule. These polymeric nanomicelles exhibited
excellent hemo- and cytocompatibility, as evaluated by in vitro hemolysis
and MTT cell viability assay, respectively, and showed superior transfection
efficiency compared to unmodified chitosan and naked DNA. The surface
of these nanomicelles can be further modified with ligands allowing
for selective targeting, enhanced cell binding, and internalization.
These nanomicelles can thus be exploited as potential nonviral gene
delivery vectors for safe and efficient gene therapy.
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Sharma, Divya; Singh, Jagdish (2017). Synthesis and Characterization of Fatty Acid Grafted
Chitosan Polymer and Their Nanomicelles for Nonviral Gene Delivery
Applications. ACS Publications. Collection. https://doi.org/10.1021/acs.bioconjchem.7b00505
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AUTHORS (2)
DS
Divya Sharma
JS
Jagdish Singh
KEYWORDS
weight distributionacyl substitutionCS polymers self-assembledacidnonviral gene delivery vectorscationic naturecell bindinggene deliveryGrafted Chitosan Polymersize exclusionMTT cell viability assaygene therapymicelle concentrationCS moleculetransfection efficiencyacyl chain lengthDNAform nanomicellesCS backbonecarboxyl groupamine groupsNonviral Gene Delivery Applications