Surface Immobilization of Viruses and Nanoparticles
Elucidates Early Events in Clathrin-Mediated Endocytosis
Version 2 2018-11-17, 00:44
Version 1 2018-09-26, 13:52
Posted on 2018-11-17 - 00:44
Clathrin-mediated endocytosis (CME)
is an important entry pathway for viruses. Here, we applied click
chemistry to covalently immobilize reovirus on surfaces to study CME
during early host–pathogen interactions. To uncouple chemical
and physical properties of viruses and determine their impact on CME
initiation, we used the same strategy to covalently immobilize nanoparticles
of different sizes. Using fluorescence live microscopy and electron
microscopy, we confirmed that clathrin recruitment depends on particle
size and discovered that the maturation into clathrin-coated vesicles
(CCVs) is independent from cargo internalization. Surprisingly, we
found that the final size of CCVs appears to be imprinted on the clathrin
coat at early stages of cargo–cell interactions. Our approach
has allowed us to unravel novel aspects of early interactions between
viruses and the clathrin machinery that influence late stages of CME
and CCVs formation. This method can be easily and broadly applied
to the field of nanotechnology, endocytosis, and virology.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Fratini, Marta; Wiegand, Tina; Funaya, Charlotta; Jiang, Zhongxiang; Shah, Pranav N. M.; Spatz, Joachim P.; et al. (2018). Surface Immobilization of Viruses and Nanoparticles
Elucidates Early Events in Clathrin-Mediated Endocytosis. ACS Publications. Collection. https://doi.org/10.1021/acsinfecdis.8b00134
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (8)
MF
Marta Fratini
TW
Tina Wiegand
CF
Charlotta Funaya
ZJ
Zhongxiang Jiang
PS
Pranav N. M. Shah
JS
Joachim P. Spatz
EC
Elisabetta Ada Cavalcanti-Adam
SB
Steeve Boulant
KEYWORDS
CCVs formationSurface Immobilizationuncouple chemicalclathrin recruitmentCME initiationcargo internalizationClathrin-Mediated Endocytosis Clathrin-mediated endocytosisclathrin-coated vesiclescovalently immobilize nanoparticlesNanoparticles Elucidatesclick chemistryelectron microscopyentry pathwaycovalently immobilize reovirusstudy CMEparticle sizeinteractionclathrin coatnovel aspectsclathrin machinery