Supplementary material from "Fluorescent component and complexation mechanism of extracellular polymeric substances during dye wastewater biotreatment by anaerobic granular sludge"

Published on 2018-02-13T11:23:36Z (GMT) by
In this study, methylene blue (MB) wastewater were biotreated by anaerobic granular sludge (AnGS) and the fluorescent components of extracellular polymeric substances (EPS) and complexation mechanism were evaluated. Based on the experimental data, the sorption of MB by live and inactivated AnGS both followed the pseudo-second-order model, and the adsorption isotherm conformed well to the Langmuir model. It was shown that the difference in the sorption of live and inactivated AnGS was not significant, indicating that the sorption is mainly a physical–chemical process and metabolically mediated diffusion is negligible. The interaction between EPS and MB was proved by three-dimensional excitation–emission matrix (3D-EEM) and synchronous fluorescence spectra. 3D-EEM indicated that Protein(PN)-like substances were the main peaks of EPS, and gradually quenched with increase of MB concentrations. According to synchronous fluorescence spectra, the main fluorescence quenching was caused by PN-like and humic-like fractions, and the type belonged to static quenching. FTIR spectra demonstrated that hydroxyl group and amino group played a major role in MB sorption.

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Li, Na; Wei, Dong; Sun, Qunqun; Han, Xiao; Du, Bin; Wei, Qin (2018): Supplementary material from "Fluorescent component and complexation mechanism of extracellular polymeric substances during dye wastewater biotreatment by anaerobic granular sludge". The Royal Society.