Supplementary material from "Expression profiling across wild and cultivated tomatoes supports the relevance of early miR482/2118 suppression for <i>Phytophthora</i> resistance"

Published on 2018-02-12T09:38:07Z (GMT) by
Plants possess a battery of specific pathogen resistance (<i>R-</i>)genes. Precise <i>R-</i>gene regulation is important in the presence and absence of a pathogen. Recently, a microRNA family, miR482/2118, was shown to regulate the expression of a major class of <i>R-</i>genes<i>,</i> nucleotide-binding site leucine-rich repeats (<i>NBS-LRRs</i>). Furthermore, RNA silencing suppressor proteins, secreted by pathogens, prevent the accumulation of miR482/2118, leading to an upregulation of <i>R-</i>genes. Despite this transcriptional release of <i>R</i>-genes, RNA silencing suppressors positively contribute to the virulence of some pathogens<i>.</i> To investigate this paradox, we analysed how the regulation of <i>NBS-LRRs</i> by miR482/2118 has been shaped by the coevolution between <i>Phytophthora infestans</i> and cultivated and wild tomatoes. We used degradome analyses and qRT-PCR to evaluate and quantify the co-expression of miR482/2118 and their <i>NBS-LRR</i> targets. Our data show that miR482/2118-mediated targeting contributes to the regulation of <i>NBS-LRRs</i> in <i>Solanum lycopersicum.</i> Based on miR482/2118 expression profiling in two additional tomato species—with different coevolutionary histories with <i>P. infestans</i>—we hypothesize that pathogen-mediated RNA silencing suppression is most effective in the interaction between <i>S. lycopersicum</i> and <i>P. infestans</i>. Furthermore, an upregulation of miR482/2118 early in the infection may increase susceptibility to <i>P. infestans</i>.

Cite this collection

de Vries, Sophie; Kukuk, Andreas; von Dahlen, Janina K.; Schnake, Anika; Kloesges, Thorsten; E. Rose, Laura (2018): Supplementary material from "Expression profiling across wild and cultivated tomatoes supports the relevance of early miR482/2118 suppression for Phytophthora resistance". The Royal Society. Collection.