Shape
Analysis of DNA–Au Hybrid Particles by
Analytical Ultracentrifugation
Version 3 2017-07-26, 08:19
Version 2 2016-08-17, 16:24
Version 1 2016-08-05, 20:04
Posted on 2017-07-26 - 08:19
Current
developments in nanotechnology have increased the demand
for nanocrystal assemblies with well-defined shapes and tunable sizes.
DNA is a particularly well-suited building block in nanoscale assemblies
because of its scalable sizes, conformational variability, and convenient
self-assembly capabilities via base pairing. In hybrid
materials, gold nanoparticles (AuNPs) can be assembled into nanoparticle
structures with programmable interparticle distances by applying appropriate
DNA sequences. However, the development of stoichiometrically defined
DNA/NP structures is still challenging since product mixtures are
frequently obtained and their purification and characterization is
the rate-limiting step in the development of DNA–NP hybrid
assemblies. Improvements in nanostructure fractionation and characterization
techniques offer great potential for nanotechnology applications in
general. This study reports the application of analytical ultracentrifugation
(AUC) for the characterization of anisotropic DNA-linked metal–crystal
assemblies. On the basis of transmission electron microscopy data
and the DNA primary sequence, hydrodynamic bead models are set up
for the interpretation of the measured frictional ratios and sedimentation
coefficients. We demonstrate that the presence of single DNA strands
on particle surfaces as well as the shape factors of multiparticle
structures in mixtures can be quantitatively described by AUC. This
study will significantly broaden the possibilities to analyze mixtures
of shape-anisotropic nanoparticle assemblies. By establishing insights
into the analysis of nanostructure mixtures based on fundamental principles
of sedimentation, a wide range of potential applications in basic
research and industry become accessible.
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Urban, Maximilan
J.; Holder, Isabelle T.; Schmid, Marius; Fernandez Espin, Vanesa; Garcia de
la Torre, Jose; Hartig, Jörg S.; et al. (2016). Shape
Analysis of DNA–Au Hybrid Particles by
Analytical Ultracentrifugation. ACS Publications. Collection. https://doi.org/10.1021/acsnano.6b01377
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AUTHORS (7)
MU
Maximilan
J. Urban
IH
Isabelle T. Holder
MS
Marius Schmid
VF
Vanesa Fernandez Espin
JG
Jose Garcia de
la Torre
JH
Jörg S. Hartig
HC
Helmut Cölfen
KEYWORDS
product mixturesscalable sizesmultiparticle structuresstudy reportsDNA strandssedimentation coefficientsshape-anisotropic nanoparticle assembliesnanoparticle structureshydrodynamic bead modelsAnalytical Ultracentrifugationbase pairingparticle surfacesshape factorsnanocrystal assembliesinterparticle distancesrate-limiting stepDNA sequencestunable sizesAUCnanoscale assembliesself-assembly capabilitiescharacterization techniques offernanostructure fractionationgold nanoparticlesbuilding blockshape Analysisnanotechnology applicationsnanostructure mixturestransmission electron microscopy data