Scan-less confocal phase imaging based on dual-comb microscopy
Posted on 2018-05-16 - 13:53
Confocal laser microscopy is a powerful tool in life science research and industrial inspection because it offers two-dimensional optical-sectioning or three-dimensional imaging capability with micrometer depth selectivity. However, the objects to be measured must be reflective, absorptive, scattering, or fluorescent because the image contrast is given by the optical intensity. If a new image contrast can be provided by the optical phase, confocal laser microscopy can be further applied for transparent non-fluorescent objects or reflective objects with nanometer unevenness by providing information on refractive index, optical thickness, or geometrical shape. Here, we report scan-less confocal dual-comb microscopy offering a phase image in addition to an amplitude image with depth selectivity by using an optical frequency comb as an optical carrier of amplitude and phase with discrete ultra-multichannels. Our technique encodes confocal amplitude and phase images of a sample onto a series of discrete modes in the optical frequency comb with well-defined amplitude and phase to establish a one-to-one correspondence between image pixels and comb modes. The technique then decodes these images from comb modes with amplitude and phase. We demonstrate confocal phase imaging with milliradian phase resolution under micrometre depth selectivity on the millisecond timescale, showing the potential to seamlessly combine confocal imaging with phase imaging by further improving the system. As a proof of concept, we demonstrate the surface topography of nanometer-scale step structures. Our technique for confocal phase imaging will find applications in nanometer-to-micrometer-to-millimeter surface topography of semiconductor objects and three-dimensional visualization of stacked living cells in culture.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Hase, Eiji; Minamikawa, Takeo; Mizuno, Takahiko; Miyamoto, Shuji; ichikawa, ryuji; HSIEH, YI-DA; et al. (2018). Scan-less confocal phase imaging based on dual-comb microscopy. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.3961767.v1
or
Select your citation style and then place your mouse over the citation text to select it.
Resource Link
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (10)
EH
Eiji Hase
TM
Takeo Minamikawa
TM
Takahiko Mizuno
SM
Shuji Miyamoto
ri
ryuji ichikawa
YH
YI-DA HSIEH
KS
Kyuki Shibuya
KS
Katsuya Sato
YN
Yoshiaki Nakajima
AA
Akifumi Asahara