Role of Trace Elements as Cofactor: An Efficient Strategy
toward Enhanced Biobutanol Production
Version 2 2018-08-15, 21:08
Version 1 2018-06-21, 20:44
Posted on 2018-08-15 - 21:08
Metabolic engineering has the potential to steadily enhance product
titers by inducing changes in metabolism. Especially, availability
of cofactors plays a crucial role in improving efficacy of product
conversion. Hence, the effect of certain trace elements was studied
individually or in combinations, to enhance butanol flux during its
biological production. Interestingly, nickel chloride (100 mg L–1) and sodium selenite (1 mg L–1)
showed a nearly 2-fold increase in solvent titer, achieving 16.13
± 0.24 and 12.88 ± 0.36 g L–1 total solvents
with yields of 0.30 and 0.33 g g–1, respectively.
Subsequently, the addition time (screened entities) was optimized
(8 h) to further increase solvent production up to 18.17 ± 0.19
and 15.5 ± 0.13 g L–1 by using nickel and selenite,
respectively. A significant upsurge in butanol dehydrogenase (BDH)
levels was observed, which reflected in improved solvent productions.
Additionally, a three-dimensional structure of BDH was also constructed
using homology modeling and subsequently docked with substrate, cofactor,
and metal ion to investigate proper orientation and molecular interactions.
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Nimbalkar, Pranhita
R.; Khedkar, Manisha A.; Parulekar, Rishikesh S.; Chandgude, Vijaya K.; Sonawane, Kailas D.; Chavan, Prakash V.; et al. (2018). Role of Trace Elements as Cofactor: An Efficient Strategy
toward Enhanced Biobutanol Production. ACS Publications. Collection. https://doi.org/10.1021/acssuschemeng.8b01611
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AUTHORS (7)
PN
Pranhita
R. Nimbalkar
MK
Manisha A. Khedkar
RP
Rishikesh S. Parulekar
VC
Vijaya K. Chandgude
KS
Kailas D. Sonawane
PC
Prakash V. Chavan
SB
Sandip B. Bankar