Revealing the Excited-State
Dynamics of the Fluorescent
Protein Dendra2
Posted on 2016-02-19 - 20:54
Green-to-red photoconversion is a reaction that occurs
in a limited
number of fluorescent proteins and that is currently mechanistically
debated. In this contribution, we report on our investigation of the
photoconvertible fluorescent protein Dendra2 by employing a combination
of pump–probe, up-conversion and single photon timing spectroscopic
techniques. Our findings indicate that upon excitation of the neutral
green state an excited state proton transfer proceeds with a time
constant of 3.4 ps between the neutral green and the anionic green
states. In concentrated solution we detected resonance energy transfer
(25 ps time constant) between green and red monomers. The time-resolved
emission spectra suggest also the formation of a super-red species,
first observed for DsRed (a red fluorescent protein from the corallimorph
species Discosoma) and consistent with
peculiar structural details present in both proteins.
CITE THIS COLLECTION
DataCiteDataCite
No result found
Fron, Eduard; Van der Auweraer, Mark; Moeyaert, Benjamien; Michiels, Jan; Mizuno, Hideaki; Hofkens, Johan; et al. (2016). Revealing the Excited-State
Dynamics of the Fluorescent
Protein Dendra2. ACS Publications. Collection. https://doi.org/10.1021/jp309219m