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REBLAB: Carbohydrate active enzymes in Lactobacillus crispatus - a possible link between the pullulanase gene and growth on glycogen

Version 3 2018-10-25, 09:43
Version 2 2018-06-22, 14:59
Version 1 2018-06-19, 07:29
Posted on 2018-10-25 - 09:43 authored by Rosanne Hertzberger
Data and text belonging to the blog post on REBLAB.org June 2018 on "Carbohydrate active enzymes in Lactobacillus crispatus - a possible link between the pullulanase gene and growth on glycogen"

see http://www.reblab.org/ongoing-experiments-log/carbohydrate-active-enzymes-in-lactobacillus-crispatus-a-possible-link-between-the-pullulanase-gene-and-growth-on-glycogen/

-protocol for dbCAN survey
-raw data dbCAN survey
-pullulanase genes of Lactobacillus crispatus RL strains.
-protocol cultivation and inoculation
-protocol NYCIII medium
-data growth assay Lactobacillus crispatus on NYCIII glycogen + Graphpad prism file used for generating the Figure.

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