Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging
Posted on 2017-04-28 - 16:41
We present a novel excitation-emission multiplexed fluorescence lifetime microscopy (FLIM) method that surpasses current FLIM techniques in multiplexing capability. The method employs Fourier multiplexing to simultaneously acquire confocal fluorescence lifetime images of multiple excitation wavelength and emission color combinations at 44,000 pixels/sec. The system is built with low-cost CW laser sources and standard PMTs with versatile spectral configuration, which can be implemented as an add-on to commercial confocal microscopes. The Fourier lifetime confocal method allows fast multiplexed FLIM imaging, which makes it possible to monitor multiple biological processes in live cells. The low cost and compatibility with commercial systems could also make multiplexed FLIM more accessible to biological research community.
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Zhao, Ming; Li, Yu; Peng, Leilei (2014). Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.3765419.v1
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FLIM techniquescell imagingexcitation wavelengthmultiplexing capabilityParallel excitation-emission multiplexed fluorescence lifetime confocal microscopyemission color combinationsPMTconfocal fluorescence lifetime imagesFourier multiplexingmultiplexed FLIM imagingCW laser sourcesresearch communityconfocal microscopesmultiplexed FLIMnovel excitation-emission multiplexed fluorescence lifetime microscopyFourier lifetime confocal method