Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging
Posted on 2017-04-28 - 16:41
We present a novel excitation-emission multiplexed fluorescence lifetime microscopy (FLIM) method that surpasses current FLIM techniques in multiplexing capability. The method employs Fourier multiplexing to simultaneously acquire confocal fluorescence lifetime images of multiple excitation wavelength and emission color combinations at 44,000 pixels/sec. The system is built with low-cost CW laser sources and standard PMTs with versatile spectral configuration, which can be implemented as an add-on to commercial confocal microscopes. The Fourier lifetime confocal method allows fast multiplexed FLIM imaging, which makes it possible to monitor multiple biological processes in live cells. The low cost and compatibility with commercial systems could also make multiplexed FLIM more accessible to biological research community.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Zhao, Ming; Li, Yu; Peng, Leilei (2014). Parallel excitation-emission multiplexed fluorescence lifetime confocal microscopy for live cell imaging. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.3765419.v1
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (3)
MZ
Ming Zhao
YL
Yu Li
LP
Leilei Peng
KEYWORDS
FLIM techniquescell imagingexcitation wavelengthmultiplexing capabilityParallel excitation-emission multiplexed fluorescence lifetime confocal microscopyemission color combinationsPMTconfocal fluorescence lifetime imagesFourier multiplexingmultiplexed FLIM imagingCW laser sourcesresearch communityconfocal microscopesmultiplexed FLIMnovel excitation-emission multiplexed fluorescence lifetime microscopyFourier lifetime confocal method