Optical Manipulation of Single Magnetic Beads in a
Microwell Array on a Digital Microfluidic Chip
Version 4 2017-07-22, 13:13
Version 3 2016-10-07, 09:13
Version 2 2016-08-30, 14:23
Version 1 2016-08-09, 17:33
Posted on 2017-07-22 - 13:13
The
detection of single molecules in magnetic microbead microwell
array formats revolutionized the development of digital bioassays.
However, retrieval of individual magnetic beads from these arrays
has not been realized until now despite having great potential for
studying captured targets at the individual level. In this paper,
optical tweezers were implemented on a digital microfluidic platform
for accurate manipulation of single magnetic beads seeded in a microwell
array. Successful optical trapping of magnetic beads was found to
be dependent on Brownian motion of the beads, suggesting a 99% chance
of trapping a vibrating bead. A tailor-made experimental design was
used to screen the effect of bead type, ionic buffer strength, surfactant
type, and concentration on the Brownian activity of beads in microwells.
With the optimal conditions, the manipulation of magnetic beads was
demonstrated by their trapping, retrieving, transporting, and repositioning
to a desired microwell on the array. The presented platform combines
the strengths of digital microfluidics, digital bioassays, and optical
tweezers, resulting in a powerful dynamic microwell array system for
single molecule and single cell studies.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Decrop, Deborah; Brans, Toon; Gijsenbergh, Pieter; Lu, Jiadi; Spasic, Dragana; Kokalj, Tadej; et al. (2016). Optical Manipulation of Single Magnetic Beads in a
Microwell Array on a Digital Microfluidic Chip. ACS Publications. Collection. https://doi.org/10.1021/acs.analchem.6b01734
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (10)
DD
Deborah Decrop
TB
Toon Brans
PG
Pieter Gijsenbergh
JL
Jiadi Lu
DS
Dragana Spasic
TK
Tadej Kokalj
FB
Filip Beunis
PG
Peter Goos
RP
Robert Puers
JL
Jeroen Lammertyn