On-Chip Microparticle and Cell Washing Using Coflow
of Viscoelastic Fluid and Newtonian Fluid
Version 2 2017-08-21, 19:21
Version 1 2017-08-17, 15:33
Posted on 2017-08-21 - 19:21
This
work investigates the on-chip washing process of microparticles
and cells using coflow configuration of viscoelastic fluid and Newtonian
fluid in a straight microchannel. By adding a small amount of biocompatible
polymers into the particle medium or cell culture medium, the induced
viscoelasticity can push particles and cells laterally from their
original medium to the coflow Newtonian medium. This behavior can
be used for particle or cell washing. First, we demonstrated on-chip
particle washing by the size-dependent migration speed using coflow
of viscoelastic fluid and Newtonian fluid. The critical particle size
for efficient particle washing was determined. Second, we demonstrated
continuous on-chip washing of Jurkat cells using coflow of viscoelastic
fluid and Newtonian fluid. The lateral migration process of Jurkat
cells along the channel length was investigated. In addition, the
cell washing quality was verified by hemocytometry and flow cytometry
with a recovery rate as high as 92.8%. Scanning spectrophotometric
measurements of the media from the two inlets and the two outlets
demonstrated that diffusion of the coflow was negligible, indicating
efficient cell washing from culture medium to phosphate-buffered saline
medium. This technique may be a safer, simpler, cheaper, and more
efficient alternative to the tedious conventional centrifugation methods
and may open up a wide range of biomedical applications.
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Yuan, Dan; Tan, Say Hwa; Sluyter, Ronald; Zhao, Qianbin; Yan, Sheng; Nguyen, N. T.; et al. (2017). On-Chip Microparticle and Cell Washing Using Coflow
of Viscoelastic Fluid and Newtonian Fluid. ACS Publications. Collection. https://doi.org/10.1021/acs.analchem.7b02671
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AUTHORS (9)
DY
Dan Yuan
ST
Say Hwa Tan
RS
Ronald Sluyter
QZ
Qianbin Zhao
SY
Sheng Yan
NN
N. T. Nguyen
JG
Jinhong Guo
JZ
Jun Zhang
WL
Weihua Li