Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care
Testing
Version 3 2018-02-10, 00:43
Version 2 2017-10-17, 19:05
Version 1 2017-09-11, 15:20
Posted on 2018-02-10 - 00:43
Alzheimer’s disease (AD) biomarkers
can reflect the neurochemical
indicators used to estimate the risk in clinical nephrology. Apolipoprotein
E (ApoE) is an early biomarker for AD in clinical diagnosis. In this
research, through bactrian camel immunization, lymphocyte isolation,
RNA extraction, and library construction, ApoE-specific Nbs with high
affinity were successfully separated from an immune phage display
nanobody library. Herein, a colorimetric immunosensor was developed
for the point-of-care testing of ApoE by layer-by-layer nanoassembly
techniques and novel nanobodies (Nbs). Using highly oriented Nbs as
the capture and detection antibodies, an on-site immunosensor was
developed by detecting the mean gray value of fade color due to the
glutaraldehyde@3-aminopropyltrimethoxysilane oxidation by H2O2. The detection limit of AopE is 0.42 pg/mL, and the
clinical analysis achieves a good performance. The novel easily operated
immunosensor may have potential application in the clinical diagnosis
and real-time monitoring for AD.
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Ren, Xiang; Yan, Junrong; Wu, Dan; Wei, Qin; Wan, Yakun (2017). Nanobody-Based Apolipoprotein E Immunosensor for Point-of-Care
Testing. ACS Publications. Collection. https://doi.org/10.1021/acssensors.7b00495
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AUTHORS (5)
XR
Xiang Ren
JY
Junrong Yan
DW
Dan Wu
QW
Qin Wei
YW
Yakun Wan
KEYWORDS
RNA extractionApolipoprotein Ecolorimetric immunosensorneurochemical indicatorsbiomarkerdetection limitADbactrian camel immunizationApoE-specific Nbsdetection antibodieslayer-by-layer nanoassembly techniquesNanobody-Based Apolipoprotein E ImmunosensorH 2 O 2lymphocyte isolationlibrary constructionpoint-of-care testingdiagnosisnovel nanobodiesphage display nanobody library