Multipoint fluorescence correlation spectroscopy using a spatial light modulator

Published on 2018-11-02T16:29:38Z (GMT) by
A multipoint holographic fluorescence correlation spectroscope (MP-hFCS) has been successfully developed. The validity of MP-hFCS is demonstrated here using diffusion measurements of fluorescent dye solutions as well as of fluorescent proteins in single cells. Furthermore, the successful detection of nuclear transport of a GFP tagged glucocorticoid receptor α indicates the possibility of being able to monitor directional molecular transport using MP-hFCS. This will provide multipoint analysis of intermolecular interaction and molecular transport in living cells. Finally, MP-hFCS can achieve multipoint diffusion measurements with high spatial and time resolution while maintaining high photon detection sensitivity.

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Yamamoto, Johtaro; Mikuni, Shintaro; Kinjo, Masataka (2018): Multipoint fluorescence correlation spectroscopy using a spatial light modulator. The Optical Society. Collection.