Multipoint fluorescence correlation spectroscopy using a spatial light modulator
Posted on 2018-11-02 - 16:29
A multipoint holographic fluorescence correlation spectroscope (MP-hFCS) has been successfully developed. The validity of MP-hFCS is demonstrated here using diffusion measurements of fluorescent dye solutions as well as of fluorescent proteins in single cells. Furthermore, the successful detection of nuclear transport of a GFP tagged glucocorticoid receptor α indicates the possibility of being able to monitor directional molecular transport using MP-hFCS. This will provide multipoint analysis of intermolecular interaction and molecular transport in living cells. Finally, MP-hFCS can achieve multipoint diffusion measurements with high spatial and time resolution while maintaining high photon detection sensitivity.
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Yamamoto, Johtaro; Mikuni, Shintaro; Kinjo, Masataka (2018). Multipoint fluorescence correlation spectroscopy using a spatial light modulator. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.4153178.v1
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AUTHORS (3)
JY
Johtaro Yamamoto
SM
Shintaro Mikuni
MK
Masataka Kinjo