Metabolite
Profiling of the Response of Burdock Roots
to Copper Stress
Version 3 2018-02-09, 14:11
Version 2 2017-10-16, 19:35
Version 1 2016-02-15, 22:36
Posted on 2018-02-09 - 14:11
Arctium lappa L. (Asteraceae), also
known as burdock, has a long history of cultivation as a dietary vegetable
worldwide. Stress in plants disrupts metabolic homeostasis and requires
adjustment of metabolic pathways. Exposure to heavy metals is one
of the most prevalent environmental stresses encountered by plants.
In this study, metabolite profiling based on 1H NMR and
GC–MS was used to obtain a holistic view of the response of
burdock roots to copper stress. The principal component analysis model
generated from the NMR data showed significant separation between
groups. Copper-treated burdock roots were characterized by increased
levels of phenols and decreased levels of primary metabolites. These
results suggest that copper stress leads to activation of the phenylpropanoid
pathway and growth inhibition. GC–MS analyses revealed increased
levels of unsaturated fatty acids and decreased levels of sterols
in the copper-treated group. Changes in metabolite concentrations
were analyzed by UPLC/QTRAP–MS, and the significances were
confirmed by two-way analysis of variance and Bonferroni’s
test. Interestingly, linoleic acid was increased about 2.7-fold, from
316 ± 64.5 to 855 ± 111 ppm, in the group treated with copper
for 6 days. This study demonstrates that metabolomic profiling is
an effective analytical approach to understanding the metabolic pathway(s)
associated with copper stress in burdock roots.
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Jung, Youngae; Ha, Miyoung; Lee, Jueun; Ahn, Yun Gyong; Kwak, Jong Hwan; Ryu, Do Hyun; et al. (2016). Metabolite
Profiling of the Response of Burdock Roots
to Copper Stress. ACS Publications. Collection. https://doi.org/10.1021/jf503193c
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AUTHORS (7)
YJ
Youngae Jung
MH
Miyoung Ha
JL
Jueun Lee
YA
Yun Gyong Ahn
JK
Jong Hwan Kwak
DR
Do Hyun Ryu
GH
Geum-Sook Hwang