Low-Cost, Readily Available 3D Microscopy Imaging System with Variable Focus Spinner

Published on 2018-11-07T15:54:39Z (GMT) by
A large open aperture in an optical system can capture high-resolution images but yields a shallow depth of field. To overcome this issue, we investigated a low-cost, readily available method for retrofitting microscopy imaging systems to achieve 3D focus scanning in this study. Specifically, a procedure for fabricating variable focus spinners with dissimilar plates was introduced, and a sequence of 12 images was captured in different focal planes. The image scale and phase were corrected, and the in-focus pixels were abstracted by employing the Laplacian operator. Finally, an all-in-focus sharp image was generated, and a depth map was obtained.

Cite this collection

Wang, Lihui; Cui, Jianjiang; Tabata, Satoshi; Ishikawa, Masatoshi (2018): Low-Cost, Readily Available 3D Microscopy Imaging System with Variable Focus Spinner. The Optical Society. Collection.