Isometric multimodal photoacoustic microscopy based on optically transparent micro-ring ultrasonic detection
Posted on 2017-04-26 - 18:56
Photoacoustic microscopy (PAM) is an attractive imaging tool that complements established optical microscopic modalities by providing additional molecular specificities through imaging optical absorption contrast. While the development of optical resolution photoacoustic microscopy (ORPAM) offers high lateral resolution, the acoustically determined axial resolution is limited due to the constraint in ultrasonic detection bandwidth. ORPAM with isometric spatial resolution along both axial and lateral directions is yet to be developed. Although recently developed sophisticated optical illumination and reconstruction methods offer improved axial resolution in ORPAM, the image acquisition procedures are rather complicated, limiting their capabilities for high-speed imaging and being easily integrated with established optical microscopic modalities. Here we report an isometric ORPAM based on an optically transparent micro-ring resonator ultrasonic detector and a commercial inverted microscope platform. Owing to the superior spatial resolution and the ease of integrating our ORPAM with established microscopic modalities, single-cell imaging with extrinsic fluorescence staining, intrinsic autofluorescence, and optical absorption can be achieved simultaneously. This technique holds promise to greatly improve the accessibility of PAM to broader biomedical researchers.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Dong, Biqin; Li, Hao; Zhang, Zhen; Zhang, Kevin; Chen, Siyu; Sun, Cheng; et al. (2015). Isometric multimodal photoacoustic microscopy based on optically transparent micro-ring ultrasonic detection. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.3755864.v1
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (7)
BD
Biqin Dong
HL
Hao Li
ZZ
Zhen Zhang
KZ
Kevin Zhang
SC
Siyu Chen
CS
Cheng Sun
HZ
Hao F. Zhang
KEYWORDS
micro-ring resonatormodalitieisometric ORPAMresolution photoacoustic microscopyextrinsic fluorescence stainingdetection Photoacoustic microscopyIsometric multimodal photoacoustic microscopymicroscope platformreconstruction methods offerimage acquisition proceduresabsorption contrastimaging tooldetection bandwidth