Integrated digital holographic microscopy based on surface plasmon resonance

We propose a novel digital holographic microscopy (DHM) by integrating surface plasmon holographic microscopy (SPHM) with reflection DHM based on the angular and polarization multiplexing techniques. Employing the double-exposure holographic interferometry, the tiny variations of specimen’s refractive index (RI) can be measured with high sensitivity by using SPHM, and meanwhile, the thickness changes of the specimen can be determined by means of reflection DHM. We experimentally monitor the volatilization process of an alcohol-water mixture droplet to verify the validity of the integrated DHM. The proposed microscopy is very promising in the biological and chemical areas for multi-information sensing of diverse specimens with low-contrast RI distributions (biomolecules, nanofluids, etc) in a dynamic and nondestructive way.