Insight into the Unique Fluorescence Quenching Property of Metal-Organic Frameworks upon DNA Binding

Published on 2017-10-16T14:03:43Z (GMT) by
Metal-organic frameworks (MOFs) have been successfully used as efficient quenchers for fluorescent DNA detection. However, the surface charge property of MOFs can inevitably affect their fluorescence quenching behavior. Herein, nanoscale MOFs (NMOFs), including MOF nanosheets and nanoparticles, have been employed to investigate the relationship between the fluorescence quenching and surface properties of NMOFs. We find that the positively and negatively charged NMOFs exhibited totally opposite fluorescence quenching properties toward negatively charged FAM-labeled double-stranded DNA (<i>ds</i>DNA). On the contrast, they show negligible influence on the sensing of positively charged TAMRA-labeled <i>ds</i>DNA. This study provides a new insight of the fluorescence quenching property of NMOFs and offers a new concept for construction of ratiometric fluorescence DNA biosensors.

Cite this collection

Wang, Huai-Song; Liu, Hai-Ling; Wang, Kang; Ding, Ya; Xu, Jing-Juan; Xia, Xing-Hua; Chen, Hong-Yuan (2017): Insight into the Unique Fluorescence Quenching Property

of Metal-Organic Frameworks upon DNA Binding. ACS Publications.

https://doi.org/10.1021/acs.analchem.7b02256

Retrieved: 09:51, Oct 23, 2017 (GMT)