In vivo laser Doppler holography of the human retina

Published on 2018-08-06T18:43:38Z (GMT) by
The eye offers a unique opportunity for non-invasive exploration of cardiovascular diseases. Optical angiography in the retina requires sensitive measurements, which hinders conventional full-field laser Doppler imaging schemes. To overcome this limitation, we used digital holography to perform laser Doppler perfusion imaging of human retina with near-infrared light. Two imaging channels with a slow and a fast CMOS camera were used simultaneously for real-time narrowband measurements, and offline wideband measurements, respectively. The beat frequency spectrum of optical interferograms recorded with the fast (up to 75 kHz) CMOS camera was analyzed by short-time Fourier transformation. Power Doppler images drawn from the Doppler power spectrum density qualitatively revealed blood flow in retinal vessels over 512 x 512 pixels covering 2.4 x 2.4 mm² on the retina with a temporal resolution down to 1.6 ms. The sensitivity to lateral motion as well as the requirements in terms of sampling frequency are discussed.

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Puyo, Léo; Paques, Michel; Fink, Mathias; Sahel, Jose; Atlan, Michael (2018): In vivo laser Doppler holography of the human retina. The Optical Society. Collection.