In Situ Nondestructive Analysis of Kalanchoe
pinnata Leaf Surface Structure by Polarization-Modulation
Infrared Reflection–Absorption Spectroscopy
Version 2 2018-11-17, 14:13
Version 1 2017-11-30, 21:43
Posted on 2018-11-17 - 14:13
The outermost surface
of the leaves of land plants is covered with
a lipid membrane called the cuticle that protects against various
stress factors. Probing the molecular-level structure of the intact
cuticle is highly desirable for understanding its multifunctional
properties. We report the in situ characterization of the surface
structure of Kalanchoe pinnata leaves using polarization-modulation
infrared reflection–absorption spectroscopy (PM-IRRAS). Without
sample pretreatment, PM-IRRAS measures the IR spectra of the leaf
cuticle of a potted K. pinnata plant. The peak position
of the CH2-related modes shows that the cuticular waxes
on the leaf surface are mainly crystalline, and the alkyl chains are
highly packed in an all-trans zigzag conformation.
The surface selection rule of PM-IRRAS revealed the average orientation
of the cuticular molecules, as indicated by the positive and negative
signals of the IR peaks. This unique property of PM-IRRAS revealed
that the alkyl chains of the waxes and the main chains of polysaccharides
are oriented almost perpendicular to the leaf surface. The nondestructive,
background-free, and environmental gas-free nature of PM-IRRAS allows
the structure and chemistry of the leaf cuticle to be studied directly
in its native environment.
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Hama, Tetsuya; Kouchi, Akira; Watanabe, Naoki; Enami, Shinichi; Shimoaka, Takafumi; Hasegawa, Takeshi (2017). In Situ Nondestructive Analysis of Kalanchoe
pinnata Leaf Surface Structure by Polarization-Modulation
Infrared Reflection–Absorption Spectroscopy. ACS Publications. Collection. https://doi.org/10.1021/acs.jpcb.7b09173
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AUTHORS (6)
TH
Tetsuya Hama
AK
Akira Kouchi
NW
Naoki Watanabe
SE
Shinichi Enami
TS
Takafumi Shimoaka
TH
Takeshi Hasegawa