Hyperspectral expansion microscopy with surface-enhanced Raman and dark-field scattering
Posted on 2018-01-10 - 19:46
Expansion microscopy (ExM) is a new technique which can yield nanoscale resolution of biological specimen on a conventional fluorescence microscope through physical expansion up to 20 times its original dimensions while preserving structural information. It however suffers from poor photostability and inherently limited multiplexing capabilities. To remedy these limitations, we propose using functionalized gold Raman nanotags as antigen markers for hyperspectral expansion imaging of mouse brain sections. We demonstrate that the nanoparticles enable multimodal imaging: bright-field, dark-field and Raman, with the added photostability, contrast enhancement and brightness due to localized surface plasmon resonances (LSPR) of gold nanoparticles.
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Artur, Camille; Womack, Tasha; Zhao, Fusheng; Eriksen, Jason; Mayerich, David; Shih, Wei-chuan (2018). Hyperspectral expansion microscopy with surface-enhanced Raman and dark-field scattering. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.3967947.v1
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AUTHORS (6)
CA
Camille Artur
TW
Tasha Womack
FZ
Fusheng Zhao
JE
Jason Eriksen
DM
David Mayerich
WS
Wei-chuan Shih