Hyperspectral expansion microscopy with surface-enhanced Raman and dark-field scattering

Published on 2018-01-10T19:46:38Z (GMT) by
Expansion microscopy (ExM) is a new technique which can yield nanoscale resolution of biological specimen on a conventional fluorescence microscope through physical expansion up to 20 times its original dimensions while preserving structural information. It however suffers from poor photostability and inherently limited multiplexing capabilities. To remedy these limitations, we propose using functionalized gold Raman nanotags as antigen markers for hyperspectral expansion imaging of mouse brain sections. We demonstrate that the nanoparticles enable multimodal imaging: bright-field, dark-field and Raman, with the added photostability, contrast enhancement and brightness due to localized surface plasmon resonances (LSPR) of gold nanoparticles.

Cite this collection

Artur, Camille; Womack, Tasha; Zhao, Fusheng; Eriksen, Jason; Mayerich, David; Shih, Wei-chuan (2018): Hyperspectral expansion microscopy with surface-enhanced Raman and dark-field scattering. The Optical Society. Collection.