Gut bacterial communities of diarrheic patients with indications of Clostridioides difficile infection
Posted on 2017-09-12 - 16:55 authored by Dominik Schneider
We present bacterial 16S rRNA gene datasets derived from stool samples
of 44 patients with diarrhea indicative of a Clostridioides difficile
infection. Patients originated from Germany, Ghana, and Indonesia. After
hospitalization and diagnosis, stool samples were taken and subjected
to DNA isolation. DNA isolations of stool samples from 35 asymptomatic
control individuals were performed. The bacterial community structure
was assessed by 16S rRNA gene analysis (V3-V4 region). Metadata from
patients and asymptomatic control individuals include gender, age,
country, presence or absence of diarrhea, concomitant diseases, and
results of the microbiological tests to diagnose C. difficile presence.
We also provide initial data analysis and an overview of the entire
dataset. After processing of raw paired-end sequencing data, reads were
merged, quality-filtered, primer sequences removed, reads truncated to
400 bp and dereplicated. Singletons were removed and remaining sequences
were sorted by cluster size, clustered at 97% sequence similarity and
chimeric sequences were discarded. Taxonomy to each operational
taxonomic units (OTU) was assigned by BLASTn searches against Silva
database 123.1 and an OTU table was constructed.
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Schneider, Dominik; Thürmer, Andrea; Gollnow, Kathleen; Lugert, Raimond; Gunka, Katrin; Groß, Uwe; et al. (2017). Gut bacterial communities of diarrheic patients with indications of Clostridioides difficile infection. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.3877591.v1
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AUTHORS (7)
AT
Andrea Thürmer
KG
Kathleen Gollnow
RL
Raimond Lugert
KG
Katrin Gunka
UG
Uwe Groß
RD
Rolf Daniel