Fast, volumetric live-cell imaging using high-resolution light-field microscopy
Posted on 2018-12-04 - 21:14
Visualizing diverse anatomical and functional traits that span many spatial scales with high spatio-temporal resolution provides insights into the fundamentals of living organisms. Light-field microscopy (LFM) has recently emerged as a scanning-free, scalable method that allows for high-speed, volumetric functional brain imaging. Given those promising applications at the tissue level, at its other extreme, this highly-scalable approach holds great potential for observing structures and dynamics in single-cell specimens. However, the challenge remains for current LFM to achieve subcellular level, near-diffraction-limited 3D spatial resolution. Here, we report high-resolution LFM (HR-LFM) for live-cell imaging with a resolution of 300-700 nm in all three dimensions, an imaging depth of several micrometers, and a volume acquisition time of milliseconds. We demonstrate the technique by imaging various cellular dynamics and structures and tracking single particles. The method may advance LFM as a particularly useful tool for understanding biological systems at multiple spatio-temporal levels.
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Li, Haoyu; Guo, Changliang; KIM-HOLZAPFEL, DEBORAH; Li, WEIYI; ALTSHULLER, YELENA; Schroeder, Bryce; et al. (2018). Fast, volumetric live-cell imaging using high-resolution light-field microscopy. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.4174514.v1
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AUTHORS (10)
HL
Haoyu Li
CG
Changliang Guo
DK
DEBORAH KIM-HOLZAPFEL
WL
WEIYI Li
YA
YELENA ALTSHULLER
BS
Bryce Schroeder
WL
WENHAO LIU
ym
yizhi meng
JF
JARROD FRENCH
KT
KEN-ICHI TAKEMARU