Extinction spectra of suspensions of microspheres:
Determination of spectral refractive index and particle
size distribution with nanometer accuracy
Posted on 2018-01-10 - 19:55
A method is presented to infer simultaneously the wavelength-dependent real refractive index
(RI) of the material of microspheres and their size distribution from extinction measurements
of particle suspensions. To derive the averaged spectral optical extinction cross section of the
microspheres from such ensemble measurements, we determined the particle concentration by
flow cytometry to an accuracy of typically 2% and adjusted the particle concentration to ensure
that perturbations due to multiple scattering are negligible. For analysis of the extinction
spectra we employ Mie theory, a series-expansion representation of the refractive index and
nonlinear numerical optimization. In contrast to other approaches, our method offers the
advantage to simultaneously determine size, size distribution and spectral refractive index of
ensembles of microparticles including uncertainty estimation.
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Gienger, Jonas; Baer, Markus; Neukammer, Joerg (2018). Extinction spectra of suspensions of microspheres:
Determination of spectral refractive index and particle
size distribution with nanometer accuracy. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.3897154.v1
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