Development of Excipient-Free Freeze-Dryable Unimolecular
Hyperstar Polymers for Efficient siRNA Silencing
Version 2 2017-06-19, 19:05
Version 1 2017-06-19, 13:05
Posted on 2017-06-19 - 19:05
We
designed a unimolecular hyperstar polymer for efficient small
interfering RNA (siRNA) delivery that can be processed under repeated
lyophilization and reconstitution without the need of any excipient.
The hyperstar polymer contains a biodegradable hyperbranched core
and is bound to siRNA through its thousands of cationic arms that
radiate from its core. The siRNA/hyperstar complexes showed siRNA
transfection efficiency that was superior to that of Lipofectamine2000
in regard to the gene for human Cu, Zn superoxide dismutase 1 (SOD1),
whose mutation causes familial amyotrophic lateral sclerosis. More
importantly, hyperstar polymers as unimolecular containers minimized
the multipolymer cross-interaction during lyophilization, and this
maintained the uniquely high transfection efficiency of the siRNA/hyperstar
complexes after repeated freeze-drying and reconstitution without
the conventional need for excipient.
CITE THIS COLLECTION
DataCite
3 Biotech
3D Printing in Medicine
3D Research
3D-Printed Materials and Systems
4OR
AAPG Bulletin
AAPS Open
AAPS PharmSciTech
Abhandlungen aus dem Mathematischen Seminar der Universität Hamburg
ABI Technik (German)
Academic Medicine
Academic Pediatrics
Academic Psychiatry
Academic Questions
Academy of Management Discoveries
Academy of Management Journal
Academy of Management Learning and Education
Academy of Management Perspectives
Academy of Management Proceedings
Academy of Management Review
Zhao, Liang; Wu, Xiang; Wang, Xiaofeng; Duan, Chunying; Wang, Hongyan; Punjabi, Amol; et al. (2017). Development of Excipient-Free Freeze-Dryable Unimolecular
Hyperstar Polymers for Efficient siRNA Silencing. ACS Publications. Collection. https://doi.org/10.1021/acsmacrolett.7b00242
or
Select your citation style and then place your mouse over the citation text to select it.
SHARE
Usage metrics
Read the peer-reviewed publication
AUTHORS (11)
LZ
Liang Zhao
XW
Xiang Wu
XW
Xiaofeng Wang
CD
Chunying Duan
HW
Hongyan Wang
AP
Amol Punjabi
YZ
Yang Zhao
YZ
Yuanwei Zhang
ZX
Zuoshang Xu
HG
Haifeng Gao
GH
Gang Han
KEYWORDS
hyperbranched coretransfection efficiencyhyperstar polymerExcipient-Free Freeze-Dryable Unimolecular Hyperstar PolymersLipofectamine 2000siRNA transfection efficiencyZn superoxide dismutase 1unimolecular hyperstar polymerSODmultipolymer cross-interactionEfficient siRNA Silencingmutation causescationic armsunimolecular containershyperstar polymers