Development of Excipient-Free Freeze-Dryable Unimolecular Hyperstar Polymers for Efficient siRNA Silencing

Published on 2017-06-19T19:05:18Z (GMT) by
We designed a unimolecular hyperstar polymer for efficient small interfering RNA (siRNA) delivery that can be processed under repeated lyophilization and reconstitution without the need of any excipient. The hyperstar polymer contains a biodegradable hyperbranched core and is bound to siRNA through its thousands of cationic arms that radiate from its core. The siRNA/hyperstar complexes showed siRNA transfection efficiency that was superior to that of Lipofectamine2000 in regard to the gene for human Cu, Zn superoxide dismutase 1 (SOD1), whose mutation causes familial amyotrophic lateral sclerosis. More importantly, hyperstar polymers as unimolecular containers minimized the multipolymer cross-interaction during lyophilization, and this maintained the uniquely high transfection efficiency of the siRNA/hyperstar complexes after repeated freeze-drying and reconstitution without the conventional need for excipient.

Cite this collection

Zhao, Liang; Wu, Xiang; Wang, Xiaofeng; Duan, Chunying; Wang, Hongyan; Punjabi, Amol; Zhao, Yang; Zhang, Yuanwei; Xu, Zuoshang; Gao, Haifeng; Han, Gang (2017): Development of Excipient-Free Freeze-Dryable Unimolecular

Hyperstar Polymers for Efficient siRNA Silencing. ACS Publications.

https://doi.org/10.1021/acsmacrolett.7b00242

Retrieved: 01:55, Aug 17, 2017 (GMT)