Data from IL-10 Directly Activates and Expands Tumor-Resident CD8⁺ T Cells without De Novo Infiltration from Secondary Lymphoid Organs

The presence of activated intratumoral T cells correlates clinically with better prognosis in patients with cancer. Although tumor vaccines can increase the number of tumor-specific CD8⁺ T cells in systemic circulation, they frequently fail to increase the number of active and tumor reactive T cells within the tumor. Here we show that treatment with the pleiotropic cytokine interleukin-10 (IL-10) induces specific activation of tumor-resident CD8⁺ T cells as well as their intratumoral expansion in several mouse tumor models. We found that inhibition of T-cell trafficking from lymphoid organs did not impair IL-10–induced tumor rejection or the activation of tumor-resident CD8⁺ T cells. Tumor-resident CD8⁺ T cells expressed elevated levels of the IL-10 receptor and were directly activated by IL-10, resulting in prominent phosphorylation of STAT3 and STAT1. Although CD4⁺ T cells, regulatory T cells, NK cells, and dendritic cells have been reported as prominent targets of IL-10 in the tumor microenvironment, we found that expression of the IL-10R was required only on CD8⁺ T cells to facilitate IL-10–induced tumor rejection as well as in situ expansion and proliferation of tumor-resident CD8 T cells. Together, our findings indicate that IL-10 activates CD8⁺ T-cell–mediated tumor control and suggest that IL-10 may represent a potential tumor immunotherapy in human patients with cancer. Cancer Res; 72(14); 3570–81. ©2012 AACR.