Core–Shell–Shell Nanoparticles for NIR
Fluorescence Imaging and NRET Swelling Reporting of Injectable or
Implantable Gels
Version 2 2019-06-26, 10:43
Version 1 2019-06-21, 19:04
Posted on 2019-06-26 - 10:43
Injectable
gels that support load are desirable for restoring the
mechanical properties of degenerated load-bearing tissue. As these
gels become increasingly sophisticated, the need to remotely image
them and monitor their swelling increases. However, imaging such gels
and monitoring their swelling using noninvasive means is challenging.
Here, we use a very low concentration of near-infrared (NIR) core–shell–shell
(CSS) reporter nanoparticles to both image and monitor swelling changes
of two load-supporting gels. The load-supporting injectable gel consisted
of covalently interlinked pH-responsive microgel (MG) particles. The
latter gel was not cytotoxic and is termed a doubly cross-linked microgel
(DX MG). Inclusion of a complementary fluorescent dye enabled ratiometric
monitoring of gel swelling changes in response to pH via nonradiative
resonance energy transfer (NRET). In addition, changes in the CSS
nanoparticle emission intensity provided a NIR-only method that could
also be used to monitor gel swelling. The gel was able to be imaged
using NIR light, after being subcutaneously injected into a tissue
model. To demonstrate versatility of our approach, CSS and the dye
were included within a model implantable gel (poly(acrylamide/acrylic
acid)) and fluorescent detection of swelling investigated. Because
the concentrations of the reporting species were too low to affect
the mechanical properties, our approach to remote gel imaging and
swelling monitoring has good potential for application in injectable
gels and implants.
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Shanks, Hannah R.; Milani, Amir H.; Lu, Dongdong; Saunders, Brian R.; Carney, Louise; Adlam, Daman J.; et al. (2019). Core–Shell–Shell Nanoparticles for NIR
Fluorescence Imaging and NRET Swelling Reporting of Injectable or
Implantable Gels. ACS Publications. Collection. https://doi.org/10.1021/acs.biomac.9b00463
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AUTHORS (9)
HS
Hannah R. Shanks
AM
Amir H. Milani
DL
Dongdong Lu
BS
Brian R. Saunders
LC
Louise Carney
DA
Daman J. Adlam
JH
Judith A. Hoyland
CB
Christopher Blount
MD
Mark Dickinson
KEYWORDS
ratiometric monitoringload-supporting injectable gelnonradiative resonance energy transfercross-linked microgelsupport loadpH-responsive microgeltissue modelreporter nanoparticlesNIR Fluorescence ImagingDX MGNRETCSS nanoparticle emission intensityNIR-only methodgel imagingload-bearing tissuelatter gelNIR lightImplantable Gels Injectable