Copying
of Mixed-Sequence RNA Templates inside Model
Protocells
Version 2 2018-04-20, 23:48
Version 1 2018-04-09, 16:20
Posted on 2018-04-20 - 23:48
The chemical replication
of RNA inside fatty acid vesicles is a
plausible step in the emergence of cellular life. On the primitive
Earth, simple protocells with the ability to import nucleotides and
short oligomers from their environment could potentially have replicated
and retained larger genomic RNA oligonucleotides within a spatially
defined compartment. We have previously shown that short 5′-phosphoroimidazolide-activated
“helper” RNA oligomers enable the nonenzymatic copying
of mixed-sequence templates in solution, using 5′-phosphoroimidazolide-activated
mononucleotides. Here, we report that citrate-chelated Mg2+, a catalyst of nonenzymatic primer extension, enhances fatty acid
membrane permeability to such short RNA oligomers up to the size of
tetramers, without disrupting vesicle membranes. In addition, selective
permeability of short, but not long, oligomers can be further enhanced
by elevating the temperature. The ability to increase the permeability
of fatty acid membranes to short oligonucleotides allows for the nonenzymatic
copying of RNA templates containing all four nucleotides inside vesicles,
bringing us one step closer to the goal of building a protocell capable
of Darwinian evolution.
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O’Flaherty, Derek
K.; Kamat, Neha P.; Mirza, Fatima N.; Li, Li; Prywes, Noam; Szostak, Jack W. (2018). Copying
of Mixed-Sequence RNA Templates inside Model
Protocells. ACS Publications. Collection. https://doi.org/10.1021/jacs.8b00639
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AUTHORS (6)
DO
Derek
K. O’Flaherty
NK
Neha P. Kamat
FM
Fatima N. Mirza
LL
Li Li
NP
Noam Prywes
JS
Jack W. Szostak