Cloning and Expression of a Nonribosomal Peptide Synthetase
to Generate Blue Rose
Version 2 2018-09-20, 18:05
Version 1 2018-09-18, 15:44
Posted on 2018-09-20 - 18:05
Rose has been entwined
with human culture and history. “Blue
rose” in English signifies unattainable hope or an impossible
mission as it does not exist naturally and is not breedable regardless
of centuries of effort by gardeners. With the knowledge of genes and
enzymes involved in flower pigmentation and modern genetic technologies,
synthetic biologists have undertaken the challenge of producing blue
rose by engineering the complicated vacuolar flavonoid pigmentation
pathway and resulted in a mauve-colored rose. A completely different
strategy presented in this study employs a dual expression plasmid
containing bacterial idgS and sfp genes. The holo-IdgS, activated by Sfp from its apo-form, is a functional
nonribosomal peptide synthetase that converts l-glutamine
into the blue pigment indigoidine. Expression of these genes upon
petal injection with agro-infiltration solution generates blue-hued
rose flowers. We envision that implementing this proof-of-concept
with obligatory modifications may have tremendous impact in floriculture
to achieve a historic milestone in rose breeding.
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Nanjaraj Urs, Ankanahalli
N.; Hu, Yiling; Li, Pengwei; Yuchi, Zhiguang; Chen, Yihua; Zhang, Yan (2018). Cloning and Expression of a Nonribosomal Peptide Synthetase
to Generate Blue Rose. ACS Publications. Collection. https://doi.org/10.1021/acssynbio.8b00187
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AUTHORS (6)
AN
Ankanahalli
N. Nanjaraj Urs
YH
Yiling Hu
PL
Pengwei Li
ZY
Zhiguang Yuchi
YC
Yihua Chen
YZ
Yan Zhang