CSE1L, a Novel Microvesicle Membrane Protein, Mediates Ras-Triggered Microvesicle Generation and Metastasis of Tumor Cells
Posted on 2012-08-29 - 05:00
Abstract Tumor-derived microvesicles are rich in metastasis-related proteases and play a role in the interactions between tumor cells and tumor microenvironment in tumor metastasis. Because shed microvesicles may remain in the extracellular environment around tumor cells, the microvesicle membrane protein may be the potential target for cancer therapy. Here we report that chromosome segregation 1-like (CSE1L) protein is a microvesicle membrane protein and is a potential target for cancer therapy. v-H-Ras expression induced extracellular signal-regulated kinase (ERK)-dependent CSE1L phosphorylation and microvesicle biogenesis in various cancer cells. CSE1L overexpression also triggered microvesicle generation, and CSE1L knockdown diminished v-H-Ras-induced microvesicle generation, matrix metalloproteinase (MMP)-2 and MMP-9 secretion and metastasis of B16F10 melanoma cells. CSE1L was preferentially accumulated in microvesicles and was located in the microvesicle membrane. Furthermore, anti-CSE1L antibody-conjugated quantum dots could target tumors in animal models. Our findings highlight a novel role of Ras-ERK signaling in tumor progression and suggest that CSE1L may be involved in the “early” and “late” metastasis of tumor cells in tumorigenesis. Furthermore, the novel microvesicle membrane protein, CSE1L, may have clinical utility in cancer diagnosis and targeted cancer therapy.
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Liao, Ching-Fong; Lin, Shu-Hui; Chen, Hung-Chang; Tai, Cheng-Jeng; Chang, Chun-Chao; Li, Li-Tzu; et al. (2018). CSE1L, a Novel Microvesicle Membrane Protein, Mediates Ras-Triggered Microvesicle Generation and Metastasis of Tumor Cells. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4236617.v1
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CSE 1L overexpressionB 16F melanoma cellsNovel Microvesicle Membrane Proteinv-H-Ras-induced microvesicle generationchromosome segregation 1-MMP -9 secretionCSE 1LCSE 1L proteintumor cellsTumor Cells Abstract Tumor-derived microvesiclesextracellular signal-regulated kinaseMediates Ras-Triggered Microvesicle Generationnovel microvesicle membrane proteinERKmicrovesicle membrane proteinanti-CSE 1L antibody-conjugated quantum dotsCSE 1L knockdowncancer therapy