CRISPR-SKIP: programmable gene splicing with single base editors
Posted on 2018-08-15 - 05:00
Abstract CRISPR gene editing has revolutionized biomedicine and biotechnology by providing a simple means to engineer genes through targeted double-strand breaks in the genomic DNA of living cells. However, given the stochasticity of cellular DNA repair mechanisms and the potential for off-target mutations, technologies capable of introducing targeted changes with increased precision, such as single-base editors, are preferred. We present a versatile method termed CRISPR-SKIP that utilizes cytidine deaminase single-base editors to program exon skipping by mutating target DNA bases within splice acceptor sites. Given its simplicity and precision, CRISPR-SKIP will be broadly applicable in gene therapy and synthetic biology.
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Gapinske, Michael; Luu, Alan; Winter, Jackson; Woods, Wendy; Kostan, Kurt; Shiva, Nikhil; et al. (2018). CRISPR-SKIP: programmable gene splicing with single base editors. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.4198871.v1
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AUTHORS (8)
MG
Michael Gapinske
AL
Alan Luu
JW
Jackson Winter
WW
Wendy Woods
KK
Kurt Kostan
NS
Nikhil Shiva
JS
Jun Song
PP
Pablo Perez-Pinera