Blood and sputum eosinophils in COPD; relationship with bacterial load
Posted on 2017-05-08 - 05:00
Abstract Background Sputum and blood eosinophil counts predict corticosteroid effects in COPD patients. Bacterial infection causes increased airway neutrophilic inflammation. The relationship of eosinophil counts with airway bacterial load in COPD patients is uncertain. We tested the hypothesis that bacterial load and eosinophil counts are inversely related. Methods COPD patients were seen at stable state and exacerbation onset. Sputum was processed for quantitative polymerase chain reaction detection of the potentially pathogenic microorganisms (PPM) H. influenzae, M. catarrhalis and S. pneumoniae. PPM positive was defined as total load ≥1 × 104copies/ml. Sputum and whole blood were analysed for differential cell counts. Results At baseline, bacterial counts were not related to blood eosinophils, but sputum eosinophil % was significantly lower in patients with PPM positive compared to PPM negative samples (medians: 0.5% vs. 1.25% respectively, p = 0.01). Patients with PPM positive samples during an exacerbation had significantly lower blood eosinophil counts at exacerbation compared to baseline (medians: 0.17 × 109/L vs. 0.23 × 109/L respectively, p = 0.008), while no blood eosinophil change was observed with PPM negative samples. Conclusions These findings indicate an inverse relationship between bacterial infection and eosinophil counts. Bacterial infection may influence corticosteroid responsiveness by altering the profile of neutrophilic and eosinophilic inflammation.
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Kolsum, Umme; Donaldson, Gavin; Singh, Richa; Barker, Bethan; Gupta, Vandana; George, Leena; et al. (2017). Blood and sputum eosinophils in COPD; relationship with bacterial load. figshare. Collection. https://doi.org/10.6084/m9.figshare.c.3774284.v1
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AUTHORS (13)
UK
Umme Kolsum
GD
Gavin Donaldson
RS
Richa Singh
BB
Bethan Barker
VG
Vandana Gupta
LG
Leena George
AW
Adam Webb
ST
Sarah Thurston
AB
Anthony Brookes
TM
Timothy McHugh
JW
Jadwiga Wedzicha
CB
Christopher Brightling
DS
Dave Singh