Antimicrobial
Chemicals Are Associated with Elevated
Antibiotic Resistance Genes in the Indoor Dust Microbiome
Version 3 2016-09-14, 13:53
Version 2 2016-09-13, 16:49
Version 1 2016-08-26, 20:44
Posted on 2016-09-07 - 00:00
Antibiotic
resistance is increasingly widespread, largely due to
human influence. Here, we explore the relationship between antibiotic
resistance genes and the antimicrobial chemicals triclosan, triclocarban,
and methyl-, ethyl-, propyl-, and butylparaben in the dust microbiome.
Dust samples from a mixed-use athletic and educational facility were
subjected to microbial and chemical analyses using a combination of
16S rRNA amplicon sequencing, shotgun metagenome sequencing, and liquid
chromatography tandem mass spectrometry. The dust resistome was characterized
by identifying antibiotic resistance genes annotated in the Comprehensive
Antibiotic Resistance Database (CARD) from the metagenomes of each
sample using the Short, Better Representative Extract Data set (ShortBRED).
The three most highly abundant antibiotic resistance genes were tet(W), blaSRT-1, and erm(B). The complete dust resistome was then compared against the measured
concentrations of antimicrobial chemicals, which for triclosan ranged
from 0.5 to 1970 ng/g dust. We observed six significant positive associations
between the concentration of an antimicrobial chemical and the relative
abundance of an antibiotic resistance gene, including one between
the ubiquitous antimicrobial triclosan and erm(X),
a 23S rRNA methyltransferase implicated in resistance to several antibiotics.
This study is the first to look for an association between antibiotic
resistance genes and antimicrobial chemicals in dust.
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Hartmann, Erica M.; Hickey, Roxana; Hsu, Tiffany; Betancourt Román, Clarisse M.; Chen, Jing; Schwager, Randall; et al. (2016). Antimicrobial
Chemicals Are Associated with Elevated
Antibiotic Resistance Genes in the Indoor Dust Microbiome. ACS Publications. Collection. https://doi.org/10.1021/acs.est.6b00262
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AUTHORS (11)
EH
Erica M. Hartmann
RH
Roxana Hickey
TH
Tiffany Hsu
CB
Clarisse M. Betancourt Román
JC
Jing Chen
RS
Randall Schwager
JK
Jeff Kline
GZ
G. Z. Brown
RH
Rolf U. Halden
CH
Curtis Huttenhower
JL
Jessica L. Green
KEYWORDS
Elevated Antibiotic Resistance GenesComprehensive Antibiotic Resistance Databasechromatography tandem mass spectrometryantibiotic resistance genesBetter Representative Extract Dataantibiotic resistance gene16 S rRNA amplicon sequencingantimicrobial chemicalsdust resistome23 S rRNA methyltransferaseIndoor Dust Microbiome Antibiotic resistanceantimicrobial chemicals triclosanshotgun metagenome sequencing