Accessible quantitative phase imaging in
confocal microscopy with sinusoidal-phase
synthetic optical holography
Posted on 2018-11-30 - 15:28
We present a technically simple implementation of quantitative phase imaging in
confocal microscopy based on synthetic optical holography with sinusoidal-phase reference
waves. Using a Mirau interference objective and low-amplitude vertical sample vibration with
a piezo-controlled stage, we record synthetic holograms on commercial confocal microscope
(Nikon, model: A1R; Zeiss: model: LSM-880), from which quantitative phase images are
reconstructed. We demonstrate our technique by stain-free imaging of cervical (HeLa), ovarian
(ES-2) cancer cells and stem cell (mHAT9a) samples. Our technique has the potential to extend
fluorescence imaging applications in confocal microscopy by providing label-free cell finding,
monitoring cell morphology as well as non-perturbing long-time observation of live cells based
on quantitative phase contrast.
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Canales, Arturo; Zhuo, Yue; Amitrano, Andrea; Kim, Minsoo; Hernandez-Aranda, Raul; Carney, P. Scott; et al. (2018). Accessible quantitative phase imaging in
confocal microscopy with sinusoidal-phase
synthetic optical holography. Optica Publishing Group. Collection. https://doi.org/10.6084/m9.figshare.c.4283600.v1
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AUTHORS (7)
AC
Arturo Canales
YZ
Yue Zhuo
AA
Andrea Amitrano
MK
Minsoo Kim
RH
Raul Hernandez-Aranda
PC
P. Scott Carney
MS
Martin Schnell