A Nano-In-Micro System for Enhanced Stem Cell Therapy
of Ischemic Diseases
Version 2 2017-08-23, 04:15
Version 1 2017-07-19, 12:12
Posted on 2017-08-23 - 04:15
Stem
cell therapy holds great potential for treating ischemic diseases.
However, contemporary methods for local stem cell delivery suffer
from poor cell survival/retention after injection. We developed a
unique multiscale delivery system by encapsulating therapeutic agent-laden
nanoparticles in alginate hydrogel microcapsules and further coentrapping
the nano-in-micro capsules with stem cells in collagen hydrogel. The
multiscale system exhibits significantly higher mechanical strength
and stability than pure collagen hydrogel. Moreover, unlike nanoparticles,
the nano-in-micro capsules do not move with surrounding body fluid
and are not taken up by the cells. This allows a sustained and localized
release of extracellular epidermal growth factor (EGF), a substance
that could significantly enhance the proliferation of mesenchymal
stem cells while maintaining their multilineage differentiation potential
via binding with its receptors on the stem cell surface. As a result,
the multiscale system significantly improves the stem cell survival
at 8 days after implantation to ∼70% from ∼4–7%
for the conventional system with nanoparticle-encapsulated EGF or
free EGF in collagen hydrogel. After injecting into the ischemic limbs
of mice, stem cells in the multiscale system facilitate tissue regeneration
to effectively restore ∼100% blood perfusion in 4 weeks without
evident side effects.
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Wang, Hai; Agarwal, Pranay; Xiao, Yichao; Peng, Hao; Zhao, Shuting; Liu, Xuanyou; et al. (2017). A Nano-In-Micro System for Enhanced Stem Cell Therapy
of Ischemic Diseases. ACS Publications. Collection. https://doi.org/10.1021/acscentsci.7b00213
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AUTHORS (10)
HW
Hai Wang
PA
Pranay Agarwal
YX
Yichao Xiao
HP
Hao Peng
SZ
Shuting Zhao
XL
Xuanyou Liu
SZ
Shenghua Zhou
JL
Jianrong Li
ZL
Zhenguo Liu
XH
Xiaoming He