A CLCA regulatory protein present in the chemosensory cilia of olfactory sensory neurons induces a Ca2+-activated Cl− current when transfected into HEK293

Published on 2017-08-11T05:00:00Z (GMT) by
Abstract Background CLCA is a family of metalloproteases that regulate Ca2+-activated Cl− fluxes in epithelial tissues. In HEK293 cells, CLCA1 promotes membrane expression of an endogenous Anoctamin 1 (ANO1, also termed TMEM16A)-dependent Ca2+-activated Cl− current. Motif architecture similarity with CLCA2, 3 and 4 suggested that they have similar functions. We previously detected the isoform CLCA4L in rat olfactory sensory neurons, where Anoctamin 2 is the principal chemotransduction Ca2+-activated Cl− channel. We explored the possibility that this protein plays a role in odor transduction. Results We cloned and expressed CLCA4L from rat olfactory epithelium in HEK293 cells. In the transfected HEK293 cells we measured a Cl−-selective Ca2+-activated current, blocked by niflumic acid, not present in the non-transfected cells. Thus, CLCA4L mimics the CLCA1 current on its ability to induce the ANO1-dependent Ca2+-activated Cl− current endogenous to these cells. By immunocytochemistry, a CLCA protein, presumably CLCA4L, was detected in the cilia of olfactory sensory neurons co-expressing with ANO2. Conclusion These findings suggests that a CLCA isoform, namely CLCA4L, expressed in OSN cilia, might have a regulatory function over the ANO2-dependent Ca2+-activated Cl− channel involved in odor transduction.

Cite this collection

Mura, Casilda; Delgado, Ricardo; Delgado, María; Restrepo, Diego; Bacigalupo, Juan (2017): A CLCA regulatory protein present in the chemosensory cilia of olfactory sensory neurons induces a Ca2+-activated Cl− current when transfected into HEK293. figshare.

https://doi.org/10.6084/m9.figshare.c.3851821

Retrieved: 14:48, Oct 19, 2017 (GMT)