4‑Cyanoindole-2′-deoxyribonucleoside (4CIN): A Universal Fluorescent Nucleoside Analogue

Published on 2018-07-11T18:49:31Z (GMT) by
The synthesis and characterization of a universal and fluorescent nucleoside, 4-cyanoindole-2′-deoxyribonucleoside (<b>4CIN</b>), and its incorporation into DNA is described. <b>4CIN</b> is a highly efficient fluorophore with quantum yields >0.90 in water. When incorporated into duplex DNA, <b>4CIN</b> pairs equivalently with native nucleobases and has uniquely high quantum yields ranging from 0.15 to 0.31 depending on sequence and hybridization contexts, surpassing that of 2-aminopurine, the prototypical nucleoside fluorophore. <b>4CIN</b> constitutes a new isomorphic nucleoside for diverse applications.

Cite this collection

Passow, Kellan

T.; Harki, Daniel A. (2018): 4‑Cyanoindole-2′-deoxyribonucleoside

(4CIN): A Universal Fluorescent Nucleoside Analogue. ACS Publications. Collection.