figshare
Browse

Michele Boiani

Dr. habil. (Biological sciences)

Muenster (NRW), Germany

I am habilitated doctor (Privatdozent) at the Westphalian Wilhelms University and group leader at the Max Planck Institute for Molecular Biomedicine, both located in Muenster (NRW, Germany). I have a keen interest in the mammalian oocyte with emphasis on the ooplasm’s ability to convert somatic nuclei from a differentiated to a toti/pluripotential state of gene expression (‘reprogramming’). I have published over 60 peer-reviewed publications in reproduction and stem cell journals, and have written numerous book chapters on nuclear reprogramming by oocytes, on embryonic stem cells, and on the effects of embryo culture conditions on pre- and postnatal health. For my research I receive support from the Deutsche Forschungsgemeinschaft (DFG) and from the Max Planck Gesellschaft (MPG). I currently serve on the Editorial Board of the International Journal of Developmental Biology and am Deputy Editor of Molecular Human Reproduction.

Publications

  • Oct4 is required for primordial germ cell survival
  • Regulatory networks in embryo-derived pluripotent stem cells
  • Nuclear reprogramming: Kinetics of cell cycle and metabolic progression as determinants of success
  • Mitochondrial physiology and gene expression analyses reveal metabolic and translational dysregulation in oocyte-induced somatic nuclear reprogramming
  • Cloned human ES cells: A great leap forward, and still needed?
  • Structural and functional integrity of spermatozoa is compromised as a consequence of acute uropathogenic E. Coli-associated epididymitis
  • The Oocyte determinants of early reprogramming
  • Maternal age effect on mouse oocytes: New biological insight from proteomic analysis
  • Retrospective analysis: reproducibility of interblastomere differences of mRNA expression in 2-cell stage mouse embryos is remarkably poor due to combinatorial mechanisms of blastomere diversification
  • Building tomorrow’s in vitro-derived germ cells on today’s solid facts
  • Cytoplasmic lattices are not linked to mouse 2-cell embryos developmental arrest
  • Subsets of cloned mouse embryos and their non-random relationship to development and nuclear reprogramming
  • Live embryo imaging to follow cell cycle and chromosomes stability after nuclear transfer
  • Differences in embryo quality are associated with differences in oocyte composition: A proteomic study in inbred mice
  • 5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming
  • Somatic cell nuclear reprogramming of mouse oocytes endures beyond reproductive decline
  • Governing cell lineage formation in cloned mouse embryos
  • DNA replication is an integral part of the mouse oocyte's reprogramming machinery
  • Effects of embryo culture media do not persist after implantation: A histological study in mice
  • Proteomic Approach to the Reprogramming Machinery of the Mouse Oocyte
  • Novel approach to sense oxygen in solution using short measurement times
  • ART culture conditions change the probability of mouse embryo gestation through defined cellular and molecular responses
  • A microfluidic system supports single mouse embryo culture leading to full-term development
  • Reprogramming of two somatic nuclei in the same ooplasm leads to pluripotent embryonic stem cells
  • Dynamic link of DNA demethylation, DNA strand breaks and repair in mouse zygotes
  • Observing and manipulating pluripotency in normal and cloned mouse embryos
  • Pluripotency and differentiation in embryos and stem cells
  • Microfluidic protocol for pre-implantation culture of single mammalian embryos: Towards an optimal culture protocol
  • Reprogramming cell fate: A scientific journey from viral enhancers to the master gene regulator Oct4 - An interview with Hans R. Schöler
  • Enhancing somatic nuclear reprogramming by Oct4 gain-of-function in cloned mouse embryos
  • International Journal of Developmental Biology: Preface
  • Proteomic Analysis of mouse oocytes reveals 28 candidate factors of the "reprogrammome"
  • Microfluidic systems for improving assisted reproductive technologies culture protocols
  • Is the perfect mouse cloning experiment in sight?
  • What scientists would like to tell you about reprogramming (if only they knew!). Preface.
  • Pavia symposium on embryos and stem cells.
  • Chromosome stability differs in cloned mouse embryos and derivative ES cells
  • Production of cloned mice by somatic cellnuclear transfer
  • Recombinant human albumin supports development of somatic cell nuclear transfer embryos in mice: Toward the establishment of a chemically defined cloning protocol
  • Variable reprogramming of the pluripotent stem cell marker Oct4 in mouse clones: Distinct developmental potentials in different culture environments
  • ATP levels in clone mouse embryos
  • Activity of the germline-specific Oct4-GFP transgene in normal and clone mouse embryos.
  • Pluripotency deficit in clones overcome by clone-clone aggregation: Epigenetic complementation?
  • Derivation of oocytes from mouse embryonic stem cells
  • Progeny from sperm obtained after ectopic grafting of neonatal mouse testes
  • Mouse Xist Expression Begins at Zygotic Genome Activation and Is Timed by a Zygotic Clock
  • Totipotency segregates between the sister blastomeres of two-cell stage mouse embryos
  • An integrated genome-wide multi-omics analysis of gene expression dynamics in the preimplantation mouse embryo
  • A framework for TRIM21-mediated protein depletion in early mouse embryos: recapitulation of Tead4 null phenotype over three days
  • Differences in blastomere totipotency in 2-cell mouse embryos are a maternal trait mediated by asymmetric mRNA distribution
  • Sperm from neonatal mammalia testes grafted in mice
  • Oct4 distribution and level in mouse clones: Consequences for pluripotency
  • The analysis of chromatin organisation allows selection of mouse antral oocytes competent for development to blastocyst
  • Gene expression and chromatin organization during mouse oocyte growth
  • Chromatin organization and meiotic non-disjunction in mouse oocytes
  • Timing of mouse Xist expression during preimplantation development
  • Analysis of aneuploidy rate in antral and ovulated mouse oocytes during female aging
  • The COP9 signalosome subunit 3 is necessary for early embryo survival by way of a stable protein deposit in mouse oocytes
  • A reproductive science perspective: deliberations on the stem cell guidelines update
  • Intracellular fraction of zona pellucida protein 3 is required for the oocyte to embryo transition in mice
  • Animal and vegetal materials of mouse oocytes segregate at first zygotic cleavage: a simple mechanism that makes the two-cell blastomeres differ reciprocally from the start

Michele Boiani's public data