Joshua Welsh

Publications

• Monolithically-integrated cytometer for measuring particle diameter in the extracellular vesicle size range using multi-angle scattering
• High Sensitivity Protein Gel Electrophoresis Label Compatible with Mass-Spectrometry
• Fluorescence and light scatter calibration allow comparisons of small particle data in standard units across different flow cytometry platforms and detector settings
• FCMPASS - Cataloguing fluorescence reference materials v2
• Towards defining reference materials for extracellular vesicle size, concentration, refractive index and epitope abundance
• Nanosight LM10
• FCMPASS - Performing and reporting calibration
• FCMPASS - Fluorescence calibration
• FCMPASS - Cataloguing light scatter reference materials
• FCMPASS - Light scatter calibration
• FCMPASS - Cataloguing fluorescence reference materials
• FCMPASS - Creating a cytometer database and datasets
• FCMPASS - Importing fcs files
• FCMPASS Protocol Collection
• FCMPASS - Acquisition and gating of light scatter reference materials
• FCMPASS - Acquisition and gating of fluorescence reference materials
• Flow Cytometer Fluorescence Voltration for FCMPASS
• Spectradyne nCS1: Sample measurement and device maintenence protocol
• Preparing EV-depleted media
• MPAPASS - Creating an MPAPASS database
• Bulk EV staining with CFDA-SE (with NAP-5 purification)
• Ultrafiltration and purification of conditioned media (Pall Jumbsosep and Izon qEV-10)
• A simple, high-throughput method of protein and label removal from extracellular vesicle samples
• MPAPASS Software Collection
• Considerations towards a roadmap for collection, handling and storage of blood extracellular vesicles
• Antigen-specific staining of EV markers with fluorochrome-conjugated antibodies
• Bulk EV staining with CFSE protein binding dye
• Astrios EQ instrument setup and sample acquisition
• Counting particles and EVs on Astrios EQ with spike in reference beads
• Detection and Sorting of Extracellular Vesicles and Viruses using nanoFACS
• Cyt-Geist: Current and Future Challenges in Cytometry: Reports of the CYTO 2019 Conference Workshops.
• Flow Virometry Quantification of Host Proteins on the Surface of HIV-1 Pseudovirus Particles
• MIFlowCyt‐EV: The Next Chapter in the Reporting and Reliability of Single Extracellular Vesicle Flow Cytometry Experiments
• Engineered Retroviruses as Fluorescent Biological Reference Particles for Small Particle Flow Cytometry
• Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence-tagged vesicles as biological reference material
• High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer
• Multiplexed assay for detection of cell culture EV surface membrane proteins v1
• Spectradyne nCS1: Sample measurement and device maintenence protocol v2
• MPAPASS - Gating flow cytometry multiplex data
• Small Particle Fluorescence and Light Scatter Calibration Using FCM PASS Software
• FCM PASS Software Aids Extracellular Vesicle Light Scatter Standardization
• Towards defining reference materials for measuring extracellular vesicle refractive index, epitope abundance, size and concentration
• Quantification of Light Scattering Detection Efficiency and Background in Flow Cytometry
• MULTIPLEX ANALYSIS OF CSF EXTRACELLULAR VESICLES OF INTRASPINAL TUMORS
• Extracellular vesicles as opportunities for integrative or focused liquid biopsy studies
• EV Translational Horizons as Viewed Across the Complex Landscape of Liquid Biopsies
• FCMPASS - Cataloguing light scatter reference materials v2
• Nanosight LM10 v1
• MIFlowCyt‐EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments
• Genome-wide methylation profiling of glioblastoma cell-derived extracellular vesicle DNA allows tumor classification
• Abstracts from the Fourth International Meeting of ISEV, ISEV2015, Washington, D.C., USA, 23-26 April 2015
• exRNA Atlas Analysis Reveals Distinct Extracellular RNA Cargo Types and Their Carriers Present across Human Biofluids
• Leukocyte extracellular vesicle concentration is inversely associated with liver fibrosis severity in NAFLD
• Technical challenges of working with extracellular vesicles
• Summary of the ISEV workshop on extracellular vesicles as disease biomarkers, held in Birmingham, UK, during December 2017
• Prospective Use of High-Refractive Index Materials for Single Molecule Detection in Flow Cytometry
• Abstracts of the UK-Russia Researcher Links Workshop: Extracellular vesicles – mechanisms of biogenesis and roles in disease pathogenesis
• Endothelial microvesicle phenotypes in individuals with non-alcoholic fatty liver disease are associated with fasting glucose, liver fibrosis and inflammation
• ISEV2018 abstract book
• Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures
• Development of an Exosome Analysis Pipeline for Precision Oncology
• Predicting Excessive Post-Operative Blood Loss in Cardiac Surgical Patients Using Platelet Microvesicles
• Extracellular Vesicle Flow Cytometry Analysis and Standardization
• The 2nd United Kingdom Extracellular Vesicle Forum Meeting Abstracts
• Microvesicles as Biomarkers in Diabetes, Obesity and Non-Alcoholic Fatty Liver Disease: Current Knowledge and Future Directions
• Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
• UK–Russia Researcher Links Workshop: extracellular vesicles – mechanisms of biogenesis and roles in disease pathogenesis, M.V. Lomonosov Moscow State University, Moscow, Russia, 1–5 March 2015
• Abstract LB-A06: Pipeline for High Throughput Analysis of Exosomes in Clinical Biofluids
• A compendium of single extracellular vesicle flow cytometry
• Extracellular vesicle refractive index derivation utilizing orthogonal characterization.
• FCMPASS Software Aids Extracellular Vesicle Light Scatter Standardization
• Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence‐tagged vesicles as biological reference material
• High‐fidelity detection and sorting of nanoscale vesicles in viral disease and cancer
• Human red blood cells release microvesicles with distinct sizes and protein composition that alter neutrophil phagocytosis
• Resource 1: Scatter Detector Setting Incrementation for FCMPASS v1
• Resource 2: Fluorescence Detector Setting Incrementation for FCMPASS v1
• Resource 3: SSC Collection Optics and Calibration v1
• Resource 4: rEV Serial Dilution v1
• Resource 5: rEV Scatter Detector Setting Incrementation v1
• Resource 6: rEV Fluorescent Detector Setting Incrementation v1
• Resource 7: rEV immunophenotyping v1
• Quantitative Flow Cytometry (qFCM) protocols for end-to-end optimization of cross-platform extracellular vesicle studies v1
• Quantitative flow cytometry enables end-to-end optimization of cross-platform extracellular vesicle studies
• Lysosomal processing of sulfatide analogues alters target NKT cell specificity and immune responses in cancer
• Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches
• MISEV2023: An updated guide to EV research and applications
• 2024, UVA EV Workshop, EV Flow Cytometry