Johannes Wolfram Debler

Publications

• Preparing Gene of interest for GateWay cloning (2 step PCR process)
• Activation of the two microRNA clusters C19MC and miR-371-3 does not play prominent role in thyroid cancer
• The novel avirulence effector AlAvr1 from Ascochyta lentis mediates host cultivar specificity of ascochyta blight in lentil
• High-molecular weight DNA extraction and small fragment removal ofAscochyta lentis v1
• Agrobacterium tumefaciens-mediated transformation and expression of GFP in Ascochyta lentis to characterize ascochyta blight disease progression in lentil
• Making electrocompetent Agrobacterium tumefaciens
• Quick Fungal DNA extraction from colonies on plates v1 (protocols.io.snzedf6)
• Electroporation of Agrobacterium
• Characterization of Growth Morphology and Pathology, and Draft Genome Sequencing of Botrytis fabae, the Causal Organism of Chocolate Spot of Faba Bean (Vicia faba L.)
• Reference Genome Assembly for Australian Ascochyta rabiei Isolate ArME14
• Targeted Disruption of Scytalone Dehydratase Gene Using Agrobacterium tumefaciens-Mediated Transformation Leads to Altered Melanin Production in Ascochyta lentis.
• An automated and combinative method for the predictive ranking of candidate effector proteins of fungal plant pathogens
• Virulence Profiles and Genome-Wide Association Study for Ascochyta lentis Isolates Collected from Australian Lentil-Growing Regions
• Preparing Gene of interest for GateWay cloning (2 step PCR process)
• Reference genome assembly for Australian Ascochyta lentis isolate Al4
• Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts
• Quick Fungal DNA extraction from colonies on plates
• Fungal gene knockout with Agrobacterium tumefaciens using Gibson assembly v1