Duarte Miguel Prazeres

Publications

• Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters DOI: 10.1016/j.bios.2013.08.037
• De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production DOI: 10.1007/s00253-012-4308-5
• Detection of ochratoxin A in wine and beer by chemiluminescence-based ELISA in microfluidics with integrated photodiodes DOI: 10.1016/j.snb.2012.10.038
• Impact of plasmid quality on lipoplex-mediated transfection DOI: 10.1002/jps.23709
• Impact of plasmid size on the purification of model plasmid DNA vaccines by phenyl membrane adsorbers DOI: 10.1016/j.chroma.2013.09.076
• Metabolic viability of Escherichia coli trapped by dielectrophoresis in microfluidics DOI: 10.1002/elps.201200292
• Streaming currents in microfluidics with integrated polarizable electrodes DOI: 10.1007/s10404-013-1153-5
• Amorphous silicon photosensors for detection of intrinsic cell fluorophores DOI: 10.1557/opl.2011.1230
• Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery DOI: 10.1016/j.jconrel.2012.01.011
• GPCR screening and drug discovery: Challenges and latest trends
• Integrated detection of intrinsic fluorophores in live microbial cells using an array of thin film amorphous silicon photodetectors DOI: 10.1016/j.bios.2012.04.029
• Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition DOI: 10.1186/1759-8753-3-1
• Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing DOI: 10.1002/biot.201100062
• Streaming current measurements in micro and nanofluidic channels for label-free multiplexed genomics diagnostics DOI: 10.1109/MEMSYS.2012.6170328
• Towards the miniaturization of GPCR-based live-cell screening assays DOI: 10.1016/j.tibtech.2012.07.004
• Validation and scale-up of plasmid DNA purification by phenyl-boronic acid chromatography DOI: 10.1002/jssc.201200225
• Electrical detection of DNA immobilization and hybridization by streaming current measurements in microchannels DOI: 10.1063/1.3658457
• Heterogeneous immunoassays in microfluidic format using fluorescence detection with integrated amorphous silicon photodiodes DOI: 10.1063/1.3553014
• Integration of thin film amorphous silicon photodetector with lab-on-chip for monitoring protein fluorescence in solution and in live microbial cells DOI: 10.1016/j.snb.2011.02.015
• Lab-on-a-chip ochratoxin A detection using competitive ELISA in microfluidics with integrated photodiode signal acquisition DOI: 10.1016/j.proeng.2011.12.297
• Microspot-based ELISA in microfluidics: Chemiluminescence and colorimetry detection using integrated thin-film hydrogenated amorphous silicon photodiodes DOI: 10.1039/c1lc20362b
• Mutation detection in plasmid-based biopharmaceuticals DOI: 10.1002/biot.201000372
• Plasmid Biopharmaceuticals: Basics, Applications, and Manufacturing DOI: 10.1002/9780470939918
• Purification of plasmid DNA from Escherichia coli ferments using anion-exchange membrane and hydrophobic chromatography DOI: 10.1002/bab.12
• Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads DOI: 10.1016/j.chroma.2011.10.004
• Towards a high-throughput drug discovery platform for the screening of GPCR targets in cells DOI: 10.1109/ENBENG.2011.6026042
• Trypanosoma brucei: Immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis DOI: 10.1016/j.exppara.2010.06.017
• Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events DOI: 10.1007/s00253-010-2671-7
• Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events DOI: 10.1007/s00253-010-2671-7
• Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography DOI: 10.1002/jmr.1068
• Characterisation of hydrogenated silicon-carbon alloy filters with different carbon composition for on-chip fluorescence detection of biomolecules DOI: 10.1016/j.sna.2010.07.016
• Clearance of host cell impurities from plasmid-containing lysates by boronate adsorption DOI: 10.1016/j.chroma.2010.02.015
• Comparative analysis of antigen-targeting sequences used in DNA vaccines DOI: 10.1007/s12033-009-9229-x
• DNA vaccines: A rational design against parasitic diseases DOI: 10.1586/erv.09.158
• Evidence for the in vivo expression of a distant downstream gene under the control of ColE1 replication origin DOI: 10.1007/s00253-009-2339-3
• Hydrophobic interaction membrane chromatography for plasmid DNA purification: Design and optimization DOI: 10.1002/jssc.200900844
• Optimization of DNA hybridization on aminopropyl-controlled pore-glass particles: Detection of non-labeled targets by picogreen staining DOI: 10.1080/00032711003731431
• Patterned functionalization layer for sub-μL DNA solid-phase immobilization and hybridization DOI: 10.1016/j.snb.2010.04.019
• Quantitation of non-amplified genomic DNA by bead-based hybridization and template mediated extension coupled to alkaline phosphatase signal amplification DOI: 10.1007/s10529-009-0149-4
• Spectral selectivity constraints in fluorescence detection of biomolecules using amorphous silicon based detectors DOI: 10.1002/pssc.200982771
• Alternatives for the intermediate recovery of plasmid DNA: Performance, economic viability and environmental impact DOI: 10.1002/biot.200800216
• Application of central composite design for DNA hybridization onto magnetic microparticles DOI: 10.1016/j.ab.2009.05.006
• Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification DOI: 10.1002/bmc.1097
• Bringing DNA vaccines closer to commercial use
• Chemiluminescent detection of horseradish peroxidase using an integrated amorphous silicon thin-film photosensor DOI: 10.1109/JSEN.2009.2027414
• Comparison of amorphous silicon photodiodes and photoconductors for detection of quantum dot biomolecular tags DOI: 10.1063/1.3259389
• Deletion formation mutations in plasmid expression vectors are unfavored by runaway amplification conditions and differentially selected under kanamycin stress DOI: 10.1016/j.jbiotec.2009.08.002
• Detection of fluorescently labeled biomolecules immobilized on a detachable substrate using an integrated amorphous silicon photodetector DOI: 10.1063/1.3122345
• Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus DOI: 10.1016/j.ijpharm.2009.05.005
• Enzymatic biosensors with integrated thin film a-Si:H photodiodes
• Evaluation of the effect of Non-B DNA structures on plasmid integrity via accelerated stability studies DOI: 10.1002/jps.21503
• Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention DOI: 10.1002/bmc.1179
• Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography DOI: 10.1002/jgm.1272
• Induction of humoral response following immunization with three different plasmid DNA vaccines against African trypanosomiasis
• Ionic Conductivity Measurements in a SiO 2 Nanochannel with PDMS Interconnects DOI: 10.1016/j.proche.2009.07.273
• Miniaturization of immunoassays using optical detection with integrated amorphous silicon photodiodes
• Plasmid purification by hydrophobic interaction chromatography using sodium citrate in the mobile phase DOI: 10.1016/j.seppur.2008.04.001
• Purification of plasmid DNA using tangential flow filtration and tandem anion-exchange membrane chromatography DOI: 10.1007/s00449-008-0284-7
• Purification of plasmid DNA with aqueous two phase systems of PEG 600 and sodium citrate/ammonium sulfate DOI: 10.1016/j.seppur.2008.01.026
• Recovery and partial purification of penicillin G acylase from E. coli homogenate and B. megaterium culture medium using an expanded bed adsorption column DOI: 10.1016/j.bej.2008.11.006
• Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high-shear vortices DOI: 10.1042/BA20080215
• Structural instability of plasmid biopharmaceuticals: challenges and implications DOI: 10.1016/j.tibtech.2009.06.004
• The effect of the shape of single, sub-ms voltage pulses on the rates of surface immobilization and hybridization of DNA DOI: 10.1088/0957-4484/20/1/015503
• The role of probe-probe interactions on the hybridization of double-stranded DNA targets onto DNA-modified magnetic microparticles DOI: 10.1007/s00216-009-2843-4
• Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis DOI: 10.1007/s00436-009-1542-6
• Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA DOI: 10.1016/j.tibtech.2008.05.005
• Amorphous silicon thin-film transistors gated through an electrolyte solution DOI: 10.1109/LED.2008.2001406
• Chemiluminescent bead-based hybridization assay for the detection of genomic DNA from E. coli in purified plasmid samples DOI: 10.1007/s00216-008-2142-5
• Colorimetric detection of molecular recognition reactions with an enzyme biolabel using a thin-film amorphous silicon photodiode on a glass substrate DOI: 10.1016/j.snb.2008.07.030
• Detection of DNA and proteins using amorphous silicon ion-sensitive thin-film field effect transistors DOI: 10.1016/j.bios.2008.05.006
• Detection of molecular tags with an integrated amorphous silicon photodetector for biological applications DOI: 10.1016/j.jnoncrysol.2007.09.096
• Electric field assisted DNA surface reactions on the sub-ms timescale
• Electrical and chemical control of surfaces for DNA immobilization and hybridization
• Fluorescence detection of DNA hybridization using an integrated thin-film amorphous silicon n-i-p photodiode
• Fluorescence detection of DNA using an amorphous silicon p-i-n photodiode DOI: 10.1063/1.2976343
• High frequency plasmid recombination mediated by 28 bp direct repeats DOI: 10.1007/s12033-008-9082-3
• Prediction of diffusion coefficients of plasmids DOI: 10.1002/bit.21626
• Recombination frequency in plasmid DNA containing direct repeats-predictive correlation with repeat and intervening sequence length DOI: 10.1016/j.plasmid.2008.06.004
• Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography DOI: 10.1016/j.ab.2007.11.005
• Chemical synthesis and crystallization of the dipeptide AcPheIleNH2 in TTAB/heptane/octanol reversed micelles DOI: 10.1016/j.jcis.2006.09.052
• Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography DOI: 10.1016/j.abb.2007.08.027
• Detection of chemiluminescence using an amorphous silicon photodiode DOI: 10.1109/JSEN.2006.890144
• Development of a candidate DNA vaccine against Maedi-Visna virus DOI: 10.1016/j.vetimm.2007.05.004
• Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography DOI: 10.1002/bmc.846
• Electrochemical detection of a dengue-related oligonucleotide sequence using ferrocenium as a hybridization indicator
• Extraction of dengue 2 plasmid DNA vaccine (pD2) from cell lysates by aqueous two-phase systems
• On the stability of plasmid DNA vectors during cell culture and purification DOI: 10.1007/s12033-007-0028-y
• On-chip control of DNA immobilization and hybridization with nanosecond electric field pulses DOI: 10.1109/SENSOR.2007.4300291
• On-chip detection of chemiluminescent biomolecules using an integrated thin film silicon photodiode
• Performance of thin-film a-Si:H microresonators in dissipative media
• pH sensitive photoconductor based on poly(para-phenylene-vinylene) DOI: 10.1016/j.snb.2006.08.005
• Preparation of plasmid DNA polyplexes from alkaline lysates by a two-step aqueous two-phase extraction process DOI: 10.1016/j.chroma.2007.06.061
• The impact of polyadenylation signals on plasmid nuclease-resistance and transgene expression DOI: 10.1002/jgm.1031
• Time-course determination of plasmid content in eukaryotic and prokaryotic cells using Real-Time PCR DOI: 10.1007/s12033-007-0007-3
• a-Si:H electrolyte-gate thin film devices for biological applications
• Humoral immune response induced in outbred CD1 mice by DNA vaccine against African Trypanosomiasis
• Label-free electronic detection of biomolecules using a-Si:H field-effect devices DOI: 10.1016/j.jnoncrysol.2006.01.058
• Optimization of isopropanol and ammonium sulfate precipitation steps in the purification of plasmid DNA DOI: 10.1021/bp060052d
• Optimization of the primary recovery of human interferon α2b from Escherichia coli inclusion bodies DOI: 10.1016/j.pep.2005.06.014
• Production of ColE1 type plasmid by Escherichia coli DH5α cultured under nonselective conditions
• Selective purification of supercoiled plasmid DNA from clarified cell lysates with a single histidine-agarose chromatography step DOI: 10.1042/BA20060082
• Thin-film silicon MEMS DNA sensors DOI: 10.1016/j.jnoncrysol.2006.01.079
• Chromatography of plasmid DNA DOI: 10.1016/j.chroma.2004.09.050
• Electric-field assisted immobilization and hybridization of DNA oligomers on thin-film microchips DOI: 10.1088/0957-4484/16/10/014
• Electrolyte-gate a-Si: H thin film transistors
• Ethanol biosensors based on alcohol oxidase DOI: 10.1016/j.bios.2004.09.030
• Purification of plasmid (pVaxLacZ) by hydrophobic interaction chromatography
• Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography DOI: 10.1016/j.chroma.2005.05.079
• Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support DOI: 10.1016/j.ab.2005.03.011
• Single base mismatch detection by microsecond voltage pulses DOI: 10.1016/j.bios.2005.02.011
• Thin film silicon microbridges for DNA detection
• Trends in dengue diagnosis DOI: 10.1002/rmv.461
• An on-chip thin film photodetector for the quantification of DNA probes and targets in microarrays.
• Application of factorial design to the study of an alcoholysis transformation promoted by cutinase immobilized on NaY zeolite and Accurel PA6 DOI: 10.1016/j.molcatb.2003.09.005
• Covalent immobilization of DNA and hybridization on microchips by microsecond electric field pulses
• Design of flowsheets for the recovery and purification of plasmids for gene therapy and DNA vaccination DOI: 10.1016/j.cep.2003.02.002
• Immobilization and hybridization by single sub-millisecond electric field pulses, for pixel-addressed DNA microarrays DOI: 10.1016/j.bios.2003.12.012
• Optoelectronic detection of DNA molecules using an amorphous silicon photodetector
• The role of polyadenylation signal secondary structures on the resistance of plasmid vectors to nucleases DOI: 10.1002/jgm.536
• Thermal and operational stabilities of Hansenula polymorpha alcohol oxidase DOI: 10.1016/j.molcatb.2003.09.001
• Translational features of human alpha 2b interferon production in Escherichia coli DOI: 10.1128/AEM.70.8.5033-5036.2004
• Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography DOI: 10.1016/S0021-9673(03)00618-6
• Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products DOI: 10.1016/j.ab.2003.07.004
• Electric field pulse assisted covalent immobilization and hybridization of DNA in the nanosecond time scale
• Electric field pulse assisted covalent immobilization and hybridization of DNA in the nanosecond time scale for genetic information analysis
• Electric-field-pulse-assisted covalent immobilization of dna in the nanosecond time scale DOI: 10.1063/1.1594839
• Horseradish peroxidase: A valuable tool in biotechnology DOI: 10.1016/S1387-2656(03)09003-3
• Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B: Using and relating two different models for describing the effect of salt and temperature on retention DOI: 10.1016/S0021-9673(03)00918-X
• Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B: Using and relating two different models for describing the effect of salt and temperature on retention DOI: 10.1016/S0021-9673(03)00918-X
• Membrane-assisted extractive bioconversions.
• Optoelectronic detection of DNA molecules using an amorphous silicon photodetector
• Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B - Application of the solvophobic theory DOI: 10.1016/S0021-9673(01)01388-7
• Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B Application of the solvophobic theory DOI: 10.1016/S0021-9673(01)01388-7
• Isolation of plasmid DNA from cell lysates by aqueous two-phase systems DOI: 10.1002/bit.10227
• Microbial stereoselective oxidation of 2-methyl-1,3-propanediol to (R)-β-hydroxyisobutyric acid in aqueous/organic biphasic systems DOI: 10.1080/10242420290020723
• Stability of a recombinant cutinase immobilized on zeolites DOI: 10.1016/S0141-0229(02)00068-6
• Thin film micro arrays with immobilized DNA for hybridization analysis
• Use of free and immobilized Pseudomonas putida cells for the reduction of a thiophene derivative in organic media DOI: 10.1016/S1381-1177(02)00186-8
• A multiphasic hollow fiber reactor for the whole-cell bioconversion of 2-methyl-1,3-propanediol to (R)-β-hydroxyisobutyric acid DOI: 10.1021/bp010015g
• Behaviour of horseradish peroxidase in AOT reversed micelles
• Conformational changes induced by immobilization of a recombinant cutinase on zeolites DOI: 10.1023/A:1016660913912
• Editorial: The Second International Conference on Protein Stabilisation, 9-12 April 2000, Lisbon
• Editorial: The Second International Conference on Protein Stabilisation, 9-12 April 2000, Lisbon
• Kinetics and modelling of an alcoholysis reaction catalyzed by cutinase immobilized on NaY zeolite DOI: 10.1016/S1381-1177(00)00050-3
• Production, purification and analysis of an experimental DNA vaccine against rabies DOI: 10.1002/jgm.218
• Purification of plasmids for gene therapy and DNA vaccination DOI: 10.1016/S1387-2656(01)07031-4
• Stability of free and immobilised peroxidase in aqueous-organic solvents mixtures DOI: 10.1016/S1381-1177(01)00017-0
• Studies on the retention of plasmid DNA and Escherichia coli nucleic acids by hydrophobic interaction chromatography.
• Study on the scale-up of human IgG3 purification using protein A affinity chromatography.
• Analysis of a Taylor-Poiseuille vortex flow reactor -- II: Reactor modeling and performance assessment using glucose-fructose isomerization as test reaction DOI: 10.1016/S0009-2509(00)00052-X
• Anion exchange purification of plasmid DNA using expanded bed adsorption DOI: 10.1023/A:1008134822673
• Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications DOI: 10.1016/S0167-7799(00)01475-X
• Kinetic and stability studies of penicillin acylase in reversed micelles
• Organic media selection for the extraction of β-hydroxyisobutyric acid produced by microbial biotransformation DOI: 10.1002/1097-4660(200007)75:7<617::AID-JCTB110>3.0.CO;2-1
• Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography DOI: 10.1002/(SICI)1097-0290(20000605)68:5<576::AID-BIT13>3.0.CO;2-5
• Quantitation of plasmid DNA in aqueous two-phase systems by fluorescence analysis DOI: 10.1023/A:1005610113677
• Scale-up of hydrophobic interaction chromatography for the purification of a DNA vaccine against rabies DOI: 10.1023/A:1005665003375
• Studies on the batch adsorption of plasmid DNA onto anion-exchange chromatographic supports DOI: 10.1021/bp0000196
• The effect of stirring and seeding on the AcPheLeuNH2 synthesis and crystallization in a reversed micellar system DOI: 10.1016/S0141-0229(00)00201-5
• Titanosilicates as supports for an enzymatic alcoholysis reaction
• Analysis and use of endogenous nuclease activities in Escherichia coil lysates during the primary isolation of plasmids for gene therapy DOI: 10.1002/(SICI)1097-0290(1999)66:3<189::AID-BIT7>3.0.CO;2-Z
• Biotransformations in two-liquid-phase systems - Production of phenylacetaldehyde by oxidation of 2-phenylethanol with acetic acid bacteria DOI: 10.1016/S0141-0229(99)00107-6
• Continuous production and simultaneous precipitation of a dipeptide in a reversed micellar membrane reactor DOI: 10.1016/S0141-0229(98)00141-0
• Development of process flow sheets for the purification of supercoiled plasmids for gene therapy applications DOI: 10.1021/bp990065+
• Enantioselective oxidation of (RS)-2-phenyl-1-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans: Simplex optimization of the biotransformation DOI: 10.1016/S0957-4166(99)00309-2
• Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: Problems and bottlenecks DOI: 10.1016/S0167-7799(98)01291-8
• Monitoring of process streams in the large-scale production and purification of plasmid DNA for gene therapy applications
• Separation and analysis of plasmid denatured forms using hydrophobic interaction chromatography DOI: 10.1006/abio.1999.4297
• Stability and stabilisation of penicillin acylase DOI: 10.1002/(SICI)1097-4660(199911)74:11<1110::AID-JCTB149>3.0.CO;2-B
• Analysis of a Taylor-Poiseuille vortex flow reactor-I: Flow patterns and mass transfer characteristics DOI: 10.1016/S0009-2509(98)00179-1
• Preparative purification of supercoiled plasmid DNA using anion- exchange chromatography DOI: 10.1016/S0021-9673(97)01254-5
• Purification of supercoiled plasmid DNA using chromatographic processes
• Zeolites as supports for an enzymatic alcoholysis reaction DOI: 10.1016/S1381-1177(98)00069-1
• A comparison of gel filtration chromatographic supports for plasmid purification
• A theoretical analogy between multistage ultrafiltration and size-exclusion chromatography DOI: 10.1016/S0009-2509(96)00467-8
• Biotransformation in organic media by enzymes and whole cells DOI: 10.1016/S0168-1656(97)00176-4
• Continuous production of isovaleraldehyde through extractive bioconversion in a hollow-fiber membrane bioreactor DOI: 10.1016/S0141-0229(96)00205-0
• Effect of the immobilization support on the hydrolytic activity of a cutinase from Fusarium solani pisi DOI: 10.1016/S0141-0229(96)00089-0
• Quantitative structure activity relationships in the synthesis of AcXYNH2, dipeptides by α-chymotrypsin in reversed micelles DOI: 10.1016/S0141-0229(97)00043-4
• Solubility studies and synthesis of AcPheLeuNH2 in reversed micellar systems
• Continuous production and in situ extraction of isovaleraldehyde in a membrane bioreactor
• Optimal design of a number of membrane reactors in series using michaelis-menten one-substrate reversible kinetics
• Phospholipase A2-catalyzed hydrolysis of lecithin in a continuous reversed-micellar membrane bioreactor
• Reversed micellar membrane bioreactor
• Zeolites as supports for enzymatic hydrolysis reactions. Comparative study of several zeolites DOI: 10.1016/1381-1177(95)00013-5
• Adsorption studies for the separation of L-tryptophan from L-serine and indole in a bioconversion medium DOI: 10.1007/BF01112999
• Concentration of BSA using a superabsorbent polymer: Process evaluation DOI: 10.1016/0168-1656(95)00014-H
• Hydrolysis of lecithin by phospholipase A2 in mixed reversed micelles of lecithin and sodium dioctyl sulphosuccinate DOI: 10.1002/jctb.280630214
• Optimal design of N CSTRs in series using Michaelis-Menten one-substrate reversible reaction
• Optimal design of N membrane reactors in series using Michaelis-Menten kinetics
• Continuous lipolysis in a reversed micellar membrane bioreactor
• Dipeptide synthesis and separation in a reversed micellar membrane reactor DOI: 10.1016/0141-0229(94)90144-9
• Enzymatic membrane bioreactors and their applications DOI: 10.1016/0141-0229(94)90030-2
• An ultrafiltration membrane bioreactor for the lipolysis of olive oil in reversed micellar media
• Colorimetric assays for the determination of dioctyl sodium sulphosuccinate and glycerol in reversed micelles DOI: 10.1007/BF00151080
• Modeling lipolysis in a reversed micellar system: Part I. Conventional batch reactor
• Modeling lipolysis in a reversed micellar system: Part II - Membrane reactor
• Protein assay in reversed micelle solutions DOI: 10.1007/BF00150901
• TEMPERATURE, PH AND MEDIA INFLUENCE ON LIPASE STABILITY
• BATCH AND CONTINUOUS LIPOLYSIS PRODUCT SEPARATION IN A REVERSED MICELLAR MEMBRANE BIOREACTOR
• Kinetics and stability of a Chromobacterium viscosum lipase in reversed micellar and aqueous media
• Capture and detection of DNA hybrids on paper via the anchoring of antibodies with fusions of carbohydrate binding modules and ZZ-domains DOI: 10.1021/ac5001288
• Characterization of the topography and wettability of english weed leaves and biomimetic replicas DOI: 10.1016/S1672-6529(14)60048-2
• Development of a phenyl membrane chromatography-based process yielding pharmaceutical grade plasmid deoxyribonucleic acid for mammalian cells transfection DOI: 10.1016/j.chroma.2014.02.024
• Engineering of Escherichia coli strains for plasmid biopharmaceutical production: Scale-up challenges DOI: 10.1016/j.vaccine.2014.02.023
• Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters DOI: 10.1007/s00253-014-5695-6
• In situ NIR spectroscopy monitoring of plasmid production processes: Effect of producing strain, medium composition and the cultivation strategy DOI: 10.1002/jctb.4431
• On the dual effect of glucose during production of pBAD/AraC-based minicircles DOI: 10.1016/j.vaccine.2014.02.035
• Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: Fermentation strategies and impact on downstream processing DOI: 10.1016/j.jbiotec.2014.06.008
• Minicircle Biopharmaceuticals – an Overview of Purification Strategies
• Multum in parvo
• Desextinção: o regresso dos mamutes
• Extreme Enhancement of Single-Molecule Fluorescence from Porphyrins Induced by Gold Nanodimer Antennas
• A Idade do Plástico
• Plasmid Copy Number of pTRKH3 in Lactococcus lactis is Increased by Modification of the repDE Ribosome‐Binding Site
• Medicina de precisão
• Conhecimento tradicional e biopirataria
• Memórias de DNA
• Agricultura celular
• A biomolecular recognition approach for the functionalization of cellulose with gold nanoparticles
• Analysis of Metallic Nanoparticles Embedded in Thin Film Semiconductors for Optoelectronic Applications
• Re-engineering of an E. coli K-12 Strain for the Efficient Production of Recombinant Human Interferon Gamma
• Production and Purification of Supercoiled Minicircles by a Combination of In Vitro Endonuclease Nicking and Hydrophobic Interaction Chromatography
• Multimodal Chromatography of Supercoiled Minicircles: A Closer Look into DNA-ligand Interactions
• Engineering of Human Mesenchymal Stem/stromal Cells (MSC) with VEGF-encoding Minicircles for Angiogenic Ex-vivo Gene Therapy
• Gold Nanotriangles as Efficient Catalysts for the Selective Oxidation of Cyclohexane
• Stability and ligand promiscuity of type A carbohydrate-binding modules are illustrated by the structure of Spirochaeta thermophila StCBM64C
• Colorimetric detection of D-dimer in a paper-based immunodetection device
• A process for supercoiled plasmid DNA purification based on multimodal chromatography
• Enhanced-Fluorescence of a Dye on DNA-Assembled Gold Nano-Dimers Discriminated by Lifetime Correlation Spectroscopy
• Stability and Ligand Promiscuity of Type A Carbohydrate-binding Modules Are Illustrated by the Structure ofSpirochaeta thermophila StCBM64C
• Vacinas, um imperativo social
• Nós e a água
• O milagre da insulina
• O biomimetismo, inovação inspirada na natureza
• Na rota da seda de aranha
• O Advento da Terapia Génica
• Density Gradient Selection of Colloidal Silver Nanotriangles for Assembling Dye-Particle Plasmophores
• DNA, genealogia e crime
• Colorimetric Detection of DNA Strands on Cellulose Microparticles Using ZZ–CBM Fusions and Gold Nanoparticles
• Monitoring Proteolytic Activity in Real Time: A New World of Opportunities for Biosensors
• Haja Sangue
• O mensageiro inesperado
• Os animais duplicados
• Affinity-Based Magnetic Particles for the Purification of Single-Stranded DNA Scaffolds for Biomanufacturing DNA-Origami Nanostructures
• Recombination efficiency measurement by real-time PCR: A strategy to evaluate ParA-mediated minicircle production
• Minicircle‐based expression of vascular endothelial growth factor in mesenchymal stromal cells from diverse human tissues
• Functionalization of Cellulose-Based Hydrogels with Bi-Functional Fusion Proteins Containing Carbohydrate-Binding Modules
• A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I
• Ionic Liquid-Polymer Nanoparticle Hybrid Systems as New Tools to Deliver Poorly Soluble Drugs
• Fluorescent dye nano-assemblies by thiol attachment directed to the tips of gold nanorods for effective emission enhancement
• Back Cover Picture: Biotechnology Journal 8/2019
• Use of Enzymes in the Downstream Processing of Biopharmaceuticals
• Fusions of a carbohydrate binding module with the small cationic hexapeptide RWRWRW confer antimicrobial properties to cellulose-based materials
• À espera de uma vacina
• Os investigadores do inútil
• O pão dos Faraós
• A patente do Dr. Chakrabarty
• Lei na Selva
• Improvement of DNA minicircle production by optimization of the secondary structure of the 5´x-UTR of ParA resolvase
• Mutation detection in plasmid-based biopharmaceuticals
• Electrical detection of DNA immobilization and hybridization by streaming current measurements in microchannels
• Heterogeneous immunoassays in microfluidic format using fluorescence detection with integrated amorphous silicon photodiodes
• Use of an E. coli pgi Knockout Strain as a Plasmid Producer
• Towards the miniaturization of GPCR-based live-cell screening assays
• DNA Instability in Bacterial Genomes: Causes and Consequences
• Use of DNA Stabilizers to Extend Plasmid Biological Activity
• Development of a phenyl membrane chromatography-based process yielding pharmaceutical grade plasmid deoxyribonucleic acid for mammalian cells transfection
• Capture and detection of DNA hybrids on paper via the anchoring of antibodies with fusions of carbohydrate binding modules and ZZ-domains
• Engineering of Escherichia coli strains for plasmid biopharmaceutical production: Scale-up challenges
• Monitoring intracellular calcium in response to GPCR activation using thin-film silicon photodiodes with integrated fluorescence filters
• Characterization of the topography and wettability of english weed leaves and biomimetic replicas
• Evidence that the insertion events of IS2 transposition are biased towards abrupt compositional shifts in target DNA and modulated by a diverse set of culture parameters
• De novo creation of MG1655-derived E. coli strains specifically designed for plasmid DNA production
• On the dual effect of glucose during production of pBAD/AraC-based minicircles
• Impact of plasmid size on the purification of model plasmid DNA vaccines by phenyl membrane adsorbers
• Metabolic viability of Escherichia coli trapped by dielectrophoresis in microfluidics
• Plasmid DNA production with Escherichia coli GALG20, a pgi-gene knockout strain: Fermentation strategies and impact on downstream processing
• Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing
• Impact of plasmid quality on lipoplex-mediated transfection
• Streaming currents in microfluidics with integrated polarizable electrodes
• Development of a recombinant fusion protein based on the dynein light chain LC8 for non-viral gene delivery
• Protein-DNA interactions define the mechanistic aspects of circle formation and insertion reactions in IS2 transposition
• Purification of plasmid DNA from Escherichia coli ferments using anion-exchange membrane and hydrophobic chromatography
• Validation and scale-up of plasmid DNA purification by phenyl-boronic acid chromatography
• Lab-on-a-chip ochratoxin A detection using competitive ELISA in microfluidics with integrated photodiode signal acquisition
• Microspot-based ELISA in microfluidics: Chemiluminescence and colorimetry detection using integrated thin-film hydrogenated amorphous silicon photodiodes
• Integration of thin film amorphous silicon photodetector with lab-on-chip for monitoring protein fluorescence in solution and in live microbial cells
• Use of ImageJ to Recover Information from Individual Cells in a G-Protein Coupled Receptor Assay
• Streaming current measurements in micro and nanofluidic channels for label-free multiplexed genomics diagnostics
• Fluorescence correlation spectroscopy study of the complexation of DNA hybrids, IgG antibody, and a chimeric protein of IgG-binding ZZ domains fused with a carbohydrate binding module
• Separation of plasmid DNA topoisomers by multimodal chromatography
• G protein-Coupled Receptors: An Overview of Signaling Mechanisms and Screening Assays
• General Aspects of Biomimetic Materials
• Detection of ochratoxin A in wine and beer by chemiluminescence-based ELISA in microfluidics with integrated photodiodes
• Amorphous silicon photosensors for detection of intrinsic cell fluorophores
• In situNIR spectroscopy monitoring of plasmid production processes: effect of producing strain, medium composition and the cultivation strategy
• Integrated detection of intrinsic fluorophores in live microbial cells using an array of thin film amorphous silicon photodetectors
• Enhancement of DNA vaccine efficacy by intracellular targeting strategies
• Plasmid Biopharmaceuticals: Basics, Applications, and Manufacturing
• Crystals of enzymatically-synthesized AcPheLeuNH2 dipeptide
• Plasmid Biopharmaceuticals
• Plasmid Biopharmaceuticals: Basics, Applications and Manufacturing
• Development of a nicking endonuclease-assisted method for the purification of minicircles
• Dynamics of Droplets of Biological Fluids on Smooth Superhydrophobic Surfaces under Electrostatic Actuation
• Towards effective non-viral gene delivery vector
• G Protein-Coupled Receptor Screening Assays
• Preparation of liposome membrane adsorbers and testing for plasmid purification
• Ionic Conductivity Measurements in a SiO 2 Nanochannel with PDMS Interconnects
• Characterisation of hydrogenated silicon-carbon alloy filters with different carbon composition for on-chip fluorescence detection of biomolecules
• DNA vaccines: A rational design against parasitic diseases
• Deletion formation mutations in plasmid expression vectors are unfavored by runaway amplification conditions and differentially selected under kanamycin stress
• Fluorescence detection of DNA using an amorphous silicon p-i-n photodiode
• Purification of plasmid DNA using tangential flow filtration and tandem anion-exchange membrane chromatography
• Recovery and partial purification of penicillin G acylase from E. coli homogenate and B. megaterium culture medium using an expanded bed adsorption column
• Studies on the adsorption of cell impurities from plasmid-containing lysates to phenyl boronic acid chromatographic beads
• The role of probe-probe interactions on the hybridization of double-stranded DNA targets onto DNA-modified magnetic microparticles
• Detection of fluorescently labeled biomolecules immobilized on a detachable substrate using an integrated amorphous silicon photodetector
• Amorphous silicon thin-film transistors gated through an electrolyte solution
• Evidence for the in vivo expression of a distant downstream gene under the control of ColE1 replication origin
• Histidine affinity chromatography of homo-oligonucleotides. Role of multiple interactions on retention
• Hydrophobic interaction membrane chromatography for plasmid DNA purification: Design and optimization
• Improvement of transfection efficiency by using supercoiled plasmid DNA purified with arginine affinity chromatography
• Purification of plasmid DNA with aqueous two phase systems of PEG 600 and sodium citrate/ammonium sulfate
• Quantitation of non-amplified genomic DNA by bead-based hybridization and template mediated extension coupled to alkaline phosphatase signal amplification
• Detection of DNA and proteins using amorphous silicon ion-sensitive thin-film field effect transistors
• Detection of molecular tags with an integrated amorphous silicon photodetector for biological applications
• Evaluation of the effect of Non-B DNA structures on plasmid integrity via accelerated stability studies
• Structural instability of plasmid biopharmaceuticals: challenges and implications
• Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography
• Binding and elution strategy for improved performance of arginine affinity chromatography in supercoiled plasmid DNA purification
• Effect of cationic liposomes/DNA charge ratio on gene expression and antibody response of a candidate DNA vaccine against Maedi Visna virus
• Plasmid purification by hydrophobic interaction chromatography using sodium citrate in the mobile phase
• Affinity chromatography approaches to overcome the challenges of purifying plasmid DNA
• Application of central composite design for DNA hybridization onto magnetic microparticles
• Comparative analysis of antigen-targeting sequences used in DNA vaccines
• Chemiluminescent bead-based hybridization assay for the detection of genomic DNA from E. coli in purified plasmid samples
• Analysis of DNA repeats in bacterial plasmids reveals the potential for recurrent instability events
• Comparison of amorphous silicon photodiodes and photoconductors for detection of quantum dot biomolecular tags
• Clearance of host cell impurities from plasmid-containing lysates by boronate adsorption
• Colorimetric detection of molecular recognition reactions with an enzyme biolabel using a thin-film amorphous silicon photodiode on a glass substrate
• Spectral selectivity constraints in fluorescence detection of biomolecules using amorphous silicon based detectors
• Stabilization of naked and condensed plasmid DNA against degradation induced by ultrasounds and high-shear vortices
• The effect of the shape of single, sub-ms voltage pulses on the rates of surface immobilization and hybridization of DNA
• Trypanosoma brucei: Immunisation with plasmid DNA encoding invariant surface glycoprotein gene is able to induce partial protection in experimental African trypanosomiasis
• Trans-sialidase from Trypanosoma brucei as a potential target for DNA vaccine development against African trypanosomiasis
• Towards a high-throughput drug discovery platform for the screening of GPCR targets in cells
• Patterned functionalization layer for sub-μL DNA solid-phase immobilization and hybridization
• Optimization of DNA hybridization on aminopropyl-controlled pore-glass particles: Detection of non-labeled targets by picogreen staining
• Chemiluminescent detection of horseradish peroxidase using an integrated amorphous silicon thin-film photosensor
• Alternatives for the intermediate recovery of plasmid DNA: Performance, economic viability and environmental impact
• Optimization of isopropanol and ammonium sulfate precipitation steps in the purification of plasmid DNA
• Chromatography of plasmid DNA
• Circular dichroism investigation of the effect of plasmid DNA structure on retention in histidine chromatography
• Design of flowsheets for the recovery and purification of plasmids for gene therapy and DNA vaccination
• Development of a candidate DNA vaccine against Maedi-Visna virus
• Immobilization and hybridization by single sub-millisecond electric field pulses, for pixel-addressed DNA microarrays
• Ethanol biosensors based on alcohol oxidase
• Optimization of the primary recovery of human interferon α2b from Escherichia coli inclusion bodies
• Label-free electronic detection of biomolecules using a-Si:H field-effect devices
• On the stability of plasmid DNA vectors during cell culture and purification
• Preparation of plasmid DNA polyplexes from alkaline lysates by a two-step aqueous two-phase extraction process
• Purification of plasmid DNA vectors by aqueous two-phase extraction and hydrophobic interaction chromatography
• Recombination frequency in plasmid DNA containing direct repeats-predictive correlation with repeat and intervening sequence length
• Single base mismatch detection by microsecond voltage pulses
• Translational features of human alpha 2b interferon production in Escherichia coli
• Selective purification of supercoiled plasmid DNA from clarified cell lysates with a single histidine-agarose chromatography step
• Separation of supercoiled and open circular plasmid DNA isoforms by chromatography with a histidine-agarose support
• Specific recognition of supercoiled plasmid DNA in arginine affinity chromatography
• Comparison of real-time polymerase chain reaction and hybridization assays for the detection of Escherichia coli genomic DNA in process samples and pharmaceutical-grade plasmid DNA products
• High frequency plasmid recombination mediated by 28 bp direct repeats
• Chemical synthesis and crystallization of the dipeptide AcPheIleNH2 in TTAB/heptane/octanol reversed micelles
• Application of factorial design to the study of an alcoholysis transformation promoted by cutinase immobilized on NaY zeolite and Accurel PA6
• Assessment of purity and quantification of plasmid DNA in process solutions using high-performance hydrophobic interaction chromatography
• Trends in dengue diagnosis
• Electric-field assisted immobilization and hybridization of DNA oligomers on thin-film microchips
• Dynamic binding capacity of plasmid DNA in histidine-agarose chromatography
• On-chip control of DNA immobilization and hybridization with nanosecond electric field pulses
• Thin-film silicon MEMS DNA sensors
• Time-course determination of plasmid content in eukaryotic and prokaryotic cells using Real-Time PCR
• Detection of chemiluminescence using an amorphous silicon photodiode
• Thermal and operational stabilities of Hansenula polymorpha alcohol oxidase
• Prediction of diffusion coefficients of plasmids
• The role of polyadenylation signal secondary structures on the resistance of plasmid vectors to nucleases
• The impact of polyadenylation signals on plasmid nuclease-resistance and transgene expression
• pH sensitive photoconductor based on poly(para-phenylene-vinylene)
• Isolation of plasmid DNA from cell lysates by aqueous two-phase systems
• Stability of a recombinant cutinase immobilized on zeolites
• Biotransformation in organic media by enzymes and whole cells
• Enantioselective oxidation of (RS)-2-phenyl-1-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans: Simplex optimization of the biotransformation
• Scale-up of hydrophobic interaction chromatography for the purification of a DNA vaccine against rabies
• Studies on the batch adsorption of plasmid DNA onto anion-exchange chromatographic supports
• Continuous production and simultaneous precipitation of a dipeptide in a reversed micellar membrane reactor
• Horseradish peroxidase: A valuable tool in biotechnology
• Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B Application of the solvophobic theory
• Kinetics and modelling of an alcoholysis reaction catalyzed by cutinase immobilized on NaY zeolite
• Electric-field-pulse-assisted covalent immobilization of dna in the nanosecond time scale
• Large-scale production of pharmaceutical-grade plasmid DNA for gene therapy: Problems and bottlenecks
• Microbial stereoselective oxidation of 2-methyl-1,3-propanediol to (R)-β-hydroxyisobutyric acid in aqueous/organic biphasic systems
• Organic media selection for the extraction of β-hydroxyisobutyric acid produced by microbial biotransformation
• Preparative purification of supercoiled plasmid DNA using anion- exchange chromatography
• Purification of a cystic fibrosis plasmid vector for gene therapy using hydrophobic interaction chromatography
• Production, purification and analysis of an experimental DNA vaccine against rabies
• Purification of plasmids for gene therapy and DNA vaccination
• Quantitation of plasmid DNA in aqueous two-phase systems by fluorescence analysis
• Use of free and immobilized Pseudomonas putida cells for the reduction of a thiophene derivative in organic media
• Conformational changes induced by immobilization of a recombinant cutinase on zeolites
• Continuous production of isovaleraldehyde through extractive bioconversion in a hollow-fiber membrane bioreactor
• Development of process flow sheets for the purification of supercoiled plasmids for gene therapy applications
• Downstream processing of plasmid DNA for gene therapy and DNA vaccine applications
• Stability and stabilisation of penicillin acylase
• A multiphasic hollow fiber reactor for the whole-cell bioconversion of 2-methyl-1,3-propanediol to (R)-β-hydroxyisobutyric acid
• A theoretical analogy between multistage ultrafiltration and size-exclusion chromatography
• Biotransformations in two-liquid-phase systems - Production of phenylacetaldehyde by oxidation of 2-phenylethanol with acetic acid bacteria
• Analysis of a Taylor-Poiseuille vortex flow reactor -- II: Reactor modeling and performance assessment using glucose-fructose isomerization as test reaction
• Analysis of a Taylor-Poiseuille vortex flow reactor-I: Flow patterns and mass transfer characteristics
• Anion exchange purification of plasmid DNA using expanded bed adsorption
• Separation and analysis of plasmid denatured forms using hydrophobic interaction chromatography
• Zeolites as supports for an enzymatic alcoholysis reaction
• Stability of free and immobilised peroxidase in aqueous-organic solvents mixtures
• The effect of stirring and seeding on the AcPheLeuNH2 synthesis and crystallization in a reversed micellar system
• Hydrophobic interaction chromatography of homo-oligonucleotides on derivatized Sepharose CL-6B: Using and relating two different models for describing the effect of salt and temperature on retention
• Analysis and use of endogenous nuclease activities in Escherichia coil lysates during the primary isolation of plasmids for gene therapy
• Zeolites as supports for enzymatic hydrolysis reactions. Comparative study of several zeolites
• Colorimetric assays for the determination of dioctyl sodium sulphosuccinate and glycerol in reversed micelles
• Hydrolysis of lecithin by phospholipase A2 in mixed reversed micelles of lecithin and sodium dioctyl sulphosuccinate
• Quantitative structure activity relationships in the synthesis of AcXYNH2, dipeptides by α-chymotrypsin in reversed micelles
• Effect of the immobilization support on the hydrolytic activity of a cutinase from Fusarium solani pisi
• Protein assay in reversed micelle solutions
• Dipeptide synthesis and separation in a reversed micellar membrane reactor
• Enzymatic membrane bioreactors and their applications
• Concentration of BSA using a superabsorbent polymer: Process evaluation
• Adsorption studies for the separation of L-tryptophan from L-serine and indole in a bioconversion medium
• Temperature, pH and Media Influence on Lipase Stability
• Batch and Continuous Lipolysis/Product Separation in a Reversed Micellar Membrane Bioreactor
• Exploring carbohydrate binding module fusions and Fab fragments in a cellulose-based lateral flow immunoassay for detection of cystatin C
• Scalable purification of single stranded DNA scaffolds for biomanufacturing DNA-origami nanostructures: exploring anion-exchange and multimodal chromatography
• Maximizing mRNA vaccine production with Bayesian optimization
• G Protein-Coupled Receptor Screening Assays. Methods and Protocols
• Colorimetric detection of D-dimer in a paper-based immunodetection device
• Exercícios e Cálculos Introdutórios em Engenharia de Bioprocessos
• Single-Particle Plasmon Sensor to Monitor Proteolytic Activity in Real Time
• Manufacturing of non-viral protein nanocages for biotechnological and biomedical applications
• Graphene oxide coatings enhance fluorescence signals in a lateral flow immunoassay for the detection of UCH-L1, a marker for trauma brain injury
• Cura por edição génica
• O fogo do génio
• Cruzamento de plantas: Tradição e ciência
• Donos de nós
• Testes em animais: o princípio do fim?
• Uma pequena revolução privada
• 50 anos de quimeras
• O homem do braço de ouro
• Os devoradores de bactérias
• O mensageiro inesperado
• Preparation of liposome membrane adsorbers and testing for plasmid purification
• O Mais Estranho dos Transplantes
• Separation of plasmid DNA topoisomers by multimodal chromatography
• Dynamics of Droplets of Biological Fluids on Smooth Superhydrophobic Surfaces under Electrostatic Actuation
• Development of a nicking endonuclease-assisted method for the purification of minicircles