Figure_3.tif (447.8 kB)
Methylation analysis of the 35S-GUS:sSat transgene in transgenic N. tabacum using McrBC PCR.
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posted on 2015-01-08, 02:41 authored by Kiran Zahid, Jian-Hua Zhao, Neil A. Smith, Ulrike Schumann, Yuan-Yuan Fang, Elizabeth S. Dennis, Ren Zhang, Hui-Shan Guo, Ming-Bo Wang(A) and (B) McrBC PCR of 35S promoter, different regions of GUS coding sequence, and Y-Sat. (C) qPCR quantification of McrBC digestion of the Y-Sat sequence shown in (B) (nt. 1-214) (left) and MUG assay of GUS activity in the transgenic plants used for the McrBC PCR analysis (right). M, DNA size marker.
