Parâmetros hematológicos, gasométricos e bioquímicos na indução da morte encefálica em ratos Wistar

Brain death (BD) is defined as the complete and irreversible loss of brain functions. In veterinary medicine, the data regarding is scarce and further studies are needed to determine more precisely the moment of BD. In human medicine, even though data is available, there is no consensus regarding the exact point of BD among the countries. This study aims to evaluate the hematologic, biochemical and gasometric parameters in Wistar rats to help develop a better understanding regarding the brain death parameters and make them more reliable, in addition to decreasing the number of misdiagnosis early. Fifteen adult male Wistar rats (Rattus norvergicus; HanUnib:WH) were randomly distributed into three groups of five animals in each: the control group (G0) with the evaluation performed before BD; and two groups (G1 and G2) which BD was induced in different moments, the first with the evaluation immediately after the induction and one hour after induction, respectively. Samples of venous and arterial blood were collected for the complete blood count and the biochemical assay, and for the blood gas assay. The samples were collected at the appropriate time according to the group each rat belonged to. Statistically significant mean values were observed (P<0,05) for segmented, where the means of G1>G2 and G0>G2; monocytes, where G2>G1 e G0>G1; creatinine, where G2>G0; AST, where G1>G0; potassium, where G2>G0; and CHCO3, where G0>G1. In addition, it was observed that there is an individual response from each organism to brain death, which makes its precise determination even more challenging.